复方绞股蓝颗粒对慢性脑缺血大鼠脑内氧化应激反应的影响中医内科学专业论文.docxVIP

PAGE PAGE 2 组:额叶皮层和海马 CA1 区偶有神经元细胞变性、死亡，正常神经元细 胞数目多。模型组:额叶皮层及海马 CA1 区有结构松散的软化灶，神经 元大量变性、死亡，神经细胞数目减少。西药组:神经元变性、死亡模 型组减轻，正常神经元细胞数目较模型组多。中药组:神经元变性、死 亡和神经细胞数目减少较西药组为略轻。 结 论： 复方绞股蓝颗粒可以明显改变脑缺血大鼠模型脑内的氧化 水平，发挥清除自由基抗氧化作用，在大鼠脑缺血引起的氧化应激中发 挥保护神经的作用。 关键词： 绞股蓝；中医药疗法；慢性脑缺血 Compound particles Gynostemma of chronic ischemic rat brain oxidative stress reaction of Abstract Objective: Observing the compound prescribe wrings a blue grain to oxidize the influence that should arouse to respond inside chronic cerebral ischemia big rat brain. Methods: A permanent bilateral carotid artery ligation (2 vo) of 40 rats with chronic cerebral ischemia in the animal model, according to figures immediately Table divided into four groups, namely, shamoperated group, model group, the Chinese Medicines Board (Compound Gynostemma ), the WM group (nimodipine), through immunohistochemical analysis, activity measurement, observation compound Gynostemma particles (Gynostemma, March 7, Astragalus) on rat brain malondialdehyde (MDA and lipid quality peroxidase), NO, SOD, and other indicators against which the effectiveness of prescription and the role of features in the original study from the oxidative stress on the basis of further evaluation of the response to improve blood circulation and the protection of the mechanism of brain cells. Provide for the future development and application of experimental basis. Results: 1. Each group cerebral ischemia in rat brain SOD changes: Compared with sham-operated group, model group of SOD activity was significantly lower P 0.01; medicine group compared with the model group, SOD activity was significantly higher P 0.01; WM group Compared with the model group with no statistical significance, that is, P 0.05. Each group cerebral ischemia in rat brain MDA changes: Compared PAGE PAGE 4 with sham-operated group, the MDA model group activity was significantly higher P 0.01; medicine group compared with the model group, MDA activity decreased significantly P 0.01; WM