当归、红芪超滤膜提取物对小鼠机体及乳鼠心肌细胞辐射损伤保护作用的实验研究-中西结合临床(心血管内科)专业论文.docxVIP

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当归、红芪超滤膜提取物对小鼠机体及乳鼠心肌细胞辐射损伤保护作用的实验研究-中西结合临床(心血管内科)专业论文.docx

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当归、红芪超滤膜提取物对小鼠机体及乳鼠心肌细胞辐射损伤保护作用的实验研究-中西结合临床(心血管内科)专业论文

当归当归、红芪超滤膜提取物对小鼠机体及乳鼠心肌细胞辐射损伤保护作用的实验研究 PAGE PAGE 10 content of bone marrow cells Abstract two Objective: To observe whether Ultra-filtration Extract from Angelica sinensis and Radix Hedysari has the protective effect on myocardial radiation injury . Methods: Primary cultures of cardiac myocytes were prepared from ventricles of 1st-to-3rd-day-old Wistar rats and cultured for 3 to 4 days. Myocardial cells were randomly divided into normal control group (C), radiation injury group (RI), low-dose drug group (LD), middle dose group(MD) and high-dose drug group (HD). The modle of radiation injury was extablished through X-ray radiation in cultured cardiomyoctes of neonatal rats after 24 hours drug effects.Then Superoxide dismutase (SOD) was measured in all groups; Bcl-2 expression was observed by Immunohistochemistry. Results: We haved obtained a good isolation of cardiomyocytes,which with purity higher than 95%.Cultured for 3 to 4 days of myocardial cells in the implementation of radiation injury, SOD was significantly lower than normal (p 0.05); LD, MD, HD group were higher than injury group (p 0.05); Bcl-2 expression was upregulate compared to (RI) group,there was significant difference (p 0.05). Conclusion: (1) This study indicate that X-ray radiation can significantly result in radiation injury in cultured Cardiomyocytes of neonatal rat;(2)Ultra-filtration Extract from Angelica sinensis, Radix Hedysari (100,000 molecular weight) can reduce the radiation damage of myocardial cells. Key Words: Angelica sinensis; Radix Hedysari; Ultra-filtration; radiation injury; Cardiomyocytes; B-cell lymphoma -2 factor 缩略词表缩略词英文全称中文名称 Bcl-2 B-cell lymphoma -2 factor B 细胞淋巴瘤-2 因子 5-Br 5 - bromo-deoxyuridine 5-溴脱氧尿嘧啶 DMSO Dimethyle sulfoxide 二亚基亚砜 DMEM Dulbecco’s modified eagle’s medium Dulbecco 改良依格氏液 LPO Lipid peroxiddation 脂质过氧化 MTT 3-(4,5-dimethylthiazol-2-yl)-2,5- Diphenyltetruroe tetrazdiumBromid 四氮唑蓝比色法 PBS Phosphate buffered saline 磷酸盐缓冲液 RI Radiation injury 辐射损伤