低频海马电刺激降低耐药性颞叶癫痫大鼠lamininβ1并抑制苔藓纤维发芽-急诊医学专业论文.docxVIP

万方数据 万方数据 与药物敏感组比较，lamininβ1 表达明显增高；海马刺激组与耐药模型组比较， laminin β1 体表明显降低（P＜0.05）。 6.经 Western blotting 法检测，laminin β1 的蛋白含量（光密度值 IOD）在 耐药对照组（0.715±0.038）较药物敏感组（0.465±0.094）表达升高；差异有统 计学意义（P＜0.05）。 结论：海马电刺激治疗可有效抑制耐药性癫痫大鼠的发作次数及级别，降低海马 组织 Lamininβ1 的含量，并抑制海马组织苔藓纤维发芽，这可能是海马电刺激治 疗耐药性癫痫的可能机制之一。 【关键词】：耐药性癫痫 海马低频电刺激 动物模型 层粘连蛋白 β1 苔藓纤维发芽 III Low-frequency hippocampal stimulation decreased the lamininβ1 level and inhibited the mossy fiber sprouting in pharmacoresistant temporal lobe epileptic rats Major: Emergency Medicine Postgraduate: Zhou Xin Supervisor: Wu Guofeng [Abstract] Objective: To establish the lithium-pilocarpine induced pharmacoresistant model of epilepsy .The low-frequency hippocampal stimulation was performed and the pathologic changes of hippocampal mossy fiber sprouting,as well as the lamininβ1 expression was abserred.So as to explore,the possible mechanism of the low-frequency hippocampal stimulation. Methods: A total of 135 healthy male SD rats were selected to prepare epileptic model by lithium-pilocarpine intraperitoneal injection.Fourteen days after the successful preparation of the model,the rats with spontaneous seizures were respectively received intraperitoneal injection with phenobarbital sodium and intragastric administration with carbamazepine. Morphological change was taken as the index. Based on the epileptic seizure level, duration, electroencephalogram(EEG) changes, and frequency, the pharmacoresistant epileptic rats were selected out according their responses to phenobarbital and carbamazepine.The pharmacoresistant epileptic rats were assigned to a drug-resistant group or a hippocampus stimulation group. The behavioral changes and EEG changes of the rats were observed to evaluate the therapeutic effect of the hippocampus stimulation at hippocampus. After the treatment was completed, the hippocampi of the rats were extracted and examined through HE staining, Timm staining, and the ultrastructures were observed under the electron microsc