傅里叶变换红外光谱应用于经染色处理的妇科肿瘤组织切片的分析-药物化学专业论文.docxVIP

重庆大学硕士学位论文英文摘要 重庆大学硕士学位论文 英文摘要 II II ABSTRACT Some of the highlights in progress of the research of fourier transform near-infrared spectroscopy in recent years were received. It could become a rapid ,precise and quantitative method of diagnosing and therapy tumor depend on the characteristic of discovering tumoring in molecule level. Usually there were two sampling-preparation methods : press method and strip method. We could receive non-interference near-infrared spectrum by directly detecting. But its hard to establish a interaction with tumor section because of the sophisticated operations. In present paper NIR was used to examine gynecologic neoplasm dyed with HE. First the differentiation between normal and tumor section 1.The IR absorb of phosphodiester When DNA in tumor cell copied ,the absorption enhanced significantly, the peak of vibration shifted from 1079cm-1 to 1083cm-1. And when nucleic acid hydrogen-banding become less, the peak was enhanced with position shifted from 1240cm-1 to 1243cm-1 which indicated that the degree of hydrogen band form by oxygen atom of the phosphodiester groups of nucleic acids was increased. The IR absorb of glycogen Proportion between normal and tumor went down. Glogcogen in tumor is less in proportion to nucleic acid . The IR absorb of c-o group in proteide Most of the hydrogen-band in tumor was destroyed withυC-O（H） band shifted from 1164cm-1 to 1175cm-1. The IR absorb of CH group Proportion between CH3 group and CH2 group went down. It probably was related with methylene chain changing. Secondly examine and confirm normal and tumor section . Keyword: FTIR , dye , tumor 重庆大学硕士学位论文目 重庆大学硕士学位论文 目 录 PAGE PAGE IV 目 录 中文摘要I 英文摘要II 1 绪 论 1 1.1 问题的提出、研究意义及国内外研究现状 1 1.1.1 问题的提出 1 1.1.2 研究意义 1 1.1.3 国内外研究现状 1 1.2 论文的研究内容及创新性 7 1.2.1 论文的研究内容 7 1.2.2 论文的创新性 8 2 理论基础 9 2.1 红外吸收光谱分析法的相关理论基础 9 2.1.1 红外吸收光谱法的基本原理 9 2.1.2 样品池和样品制备 12 2.1.3 红外光谱仪的结构和工作原理 14 2.1.4 傅里叶变换红外光谱仪的结构和工作原理 15 2.2 病理切片染色技术的基本方法和原理 17 2.2