《利用BC5F3群体选择光周期敏感性基因交换单株的研究》-毕业论文.docVIP

w w 利用BC5F3群体选择光周期敏感性基因交换单株的研究 摘　要 玉米是世界上分布最广泛的粮食作物之一，也是全世界产量最高的粮食作物。长期以来我国采用的育种方法使得我国玉米的种质基础相对狭窄与脆弱，利用热带和亚热带的玉米种质资源是一个很好的选择。但热带、亚热带的玉米种质具有很强的光走起敏感性，引入到温带往往会表现出各种明显的不适应现象。因此对玉米光周期敏感型基因的研究具有很强的理论和实际意义。 以光周期钝感自交系黄早4和光周期敏感自交系CML 288为亲本杂交所衍生重组自交系为材料，在长日照环境下鉴定其表现型，对其抽雄期、散粉期、吐丝期等进行数据记录并作出分析，在一万多株表现类型中选择极端表现的单株，对其DNA采用SDS法提取各株玉米的叶片DNA,通过变性聚丙烯酰胺凝胶电泳电泳检测等方法鉴定其基因型，并同时利用EXCEL数据分析方法、从中选择交换单株。以便利用选择出的交换单株对玉米光周期敏感性主效QTLqDPS10-1的精细定位。 经过对实验数据处理和对样品DNA的提取和目标片段的PCR扩增，最终确定极端早熟单株为272-1-1、278-1-1、287-2-2、289-1-2、290-1-1、290-1-2、328-1-1、409-1-2、410-1-3、410-2-2；极端晚熟单株为562-1-2、539-2-1、539-2-3、538-2-1、530-1-4、527-1-3、521-2-3、432-2-3、435-2-3、444-2-4。交换单株为276-2、315-1、341-1、464-1、295-1、360-1、320-2、402-2、446-1、407-1、430-2、464-1、524-2、560-1号单株，交换单元位于W118和W129之间。 关键词：玉米，光周期，交换单株，敏感性 USE BC5F3 GROUP SELECTION LIGHT CYCLE SENSITIVITY PLANTS OF EXCHANGING GENES ABSTRACT Corn is the most widely distributed food crops, and also one of the worlds highest yield food crops. In our country for a long time the breeding method makes our corn germplasm relatively narrow and fragile foundation, use of tropical and subtropical maize germplasm resources is a very good choice. But the tropical and subtropical maize germplasm has the very strong light go up sensitivity, introduced to the temperate tend to show various obvious does not adapt the phenomenon. So the corn light cycle sensitive type gene research has a strong theoretical and practical significance. o light cycle and insensitive as early as 4 and light cycle breeding excellent inbred lines yellow sensitive CML 288 for parents breeding excellent inbred lines derived from BC5F3 generation of hybrid breeding excellent inbred lines for restructuring materials, using the choice of target sites heterozygous yield per plant, it make the yield of the harvest for F2 seed in Luoyang academy of farm planting, each a different type of seed independent in plant line, in long daylight environment appraisal its phenotype, to its tasseling, powder period, such as