以胶束电动层析法与微乳液电动层析法分析大黄中之Anthraquinones.PDFVIP

行政院國家科學委員會專題研究計畫 成果報告 以膠束電動層析法與微乳液電動層析法分析大黃中之 計畫類別：個別型計畫 計畫編號：NSC91-2320-B-002-135- 執行期間：91年08月01日至92年07月31日 執行單位：國立臺灣大學醫學院藥學系暨研究所 計畫主持人：孫紹文 報告類型：完整報告 處理方式：本計畫可公開查詢 中 華 民 92年10月27日 國 Analysis of nine rhubarb anthraquinones and bianthrones by microemulsion electrokinetic chromatography Shao-Wen Sun School of Pharmacy, College of Medicine, National Taiwan University, 1 Jen-Ai Road Sec. 1, Taipei, Taiwan 1. Introduction Rhubarb, a well-known Chinese herbal medicine, has long been used in oriental preparations. Pharmacological actions of rhubarb include laxative, antibacterial, hemostatic, and antispasmodic [1-3]. Anthraquinones and bianthrones were found to be the major active principles. The British Pharmacopoeia and European Pharmacopoeia prescribe spectrometric methods to determine the amount of total hydroxyanthracene derivatives in rhubarb preparations [4,5]. These methods suffer from the lack of specificity for individual anthraquinones. Due to its high resolution, short analysis time, and low solvent and sample consumption, capillary electrophoresis (CE) has become a powerful tool in natural product analysis. tion micellar electrokinetic chromatogr charged and neutral solutes simu nanometer-sized droplets of an i layers. By the addition of a s han MEKC [8] . In the previous study of our laboratory, nine anthraquinones and bianthrones components in rhubarb, namely, aloe-emodin, emodin, chrysophanol, physcion, rhein, sennoside A, sennoside B, sennidin