mALBβ2MGα1MG检测对糖尿病肾脏早期损害临床意义.docVIP

mALBβ2MGα1MG检测对糖尿病肾脏早期损害临床意义 　　[摘要] 目的:探讨尿微量清蛋白(mALB)、β2-微球蛋白(β2-MG)和α1-微球蛋白(α1-MG)检测对糖尿病肾脏早期损害的诊断价值。方法:根据尿蛋白阴、阳性分组,设正常对照组,应用颗粒增强免疫透射比浊法检测晨尿3种微量蛋白。结果:糖尿病尿蛋白阳性组尿mALB、β2-MG和α1-MG含量均显著高于糖尿病尿蛋白阴性组,两组均显著高于正常对照组。结论:检测尿mALB、β2-MG和α1-MG对诊断糖尿病肾病早期损害具有重要的诊断价值和临床意义。 　　[关键词] 微量蛋白;糖尿病;肾脏早期损害 　　[中图分类号] R781.6+4[文献标识码]A [文章编号]1673-7210(2009)07(a)-013-02 　　 　　The clinical significance of detecting mALB, β2-MG and α1-MG in the early damage of diabetic kidney 　　ZHU Hongming1, LIAN Haiyan2 　　(1.Department of Laboratory, Jilin City Hospital, Jilin 132001, China; 2.Department of Blood Transfusion, Jilin Chemical Industrial Company Workers Hospital, Jilin 132000, China) 　　[Abstract] Objective: To explore the diagnostic value of detecting urinary microalbumin (mALB), β2-microglobulin (β2-MG) and α1-microglobulin (α1-MG) in the early damage of diabetic kidney. Methods: Cases were divided according to urinary protein negative and positive results, and normal control group was also set up, particle reinforced transmission immunoturbidimetry assay was applied to detect three kinds of trace protein in morning urine. Results: mALB, β2-MG and α1-MG content of diabetic urinary protein positive group were significantly higher than those of diabetic urinary protein negative group, the two groups were significantly higher than the normal control group. Conclusion: Detection of urinary mALB, β2-MG and α1-MG has important diagnostic value and clinical significance in the diagnosis of early damage of diabetic nephropathy. 　　[Key words] Trace protein; Diabetes; Early damage of kidney 　　 　　糖尿病肾病(DN)是糖尿病的严重并发症,其在1型糖尿病中的发生率约为35%,在2型糖尿病中的发生率约为25%。近年临床研究表明,糖尿病肾病早期,患者都有不同程度的尿微量蛋白排出增加,如尿微量清蛋白(mALB)、β2-微球蛋白(β2-MG)和α1-微球蛋白(α1-MG)等[1]。本文采用日立7170全自动生化分析仪,应用颗粒增强免疫透射比浊法检测了90例患者尿mALB、β2-MG和α1-MG含量,探讨3种微量蛋白在糖尿病肾病早期损害中的诊断价值。 　　1 资料与方法 　　1.1 一般资料 　　①临床确诊为糖尿病、尿蛋白阴性组30例,年龄(43±14)岁;②临床确诊为糖尿病、尿蛋白阳性组30例,年龄(45±10)岁;③正常对照组30例,年龄(43±9)岁。 　　1.2 尿微量蛋白检测 　　留取晨尿送检,尿mALB、β2-MG和α1-MG采用日立7170全自动生化分析仪,应用颗粒增强免疫透射比浊法,试剂盒