病毒学实验技术Techniques in Virology课件.pptx

Techniques in VirologyThis slide is download on /(中国病毒学自由学术论坛)Shared by Victor (wwwkkk83)Original slides (PPT files) are download on the internet.Thanks for authors of these slides.viruses are usuallydetected by indirect methods(i) multiplication in a suitable culture system and detection of the virus by the effects it causes; (ii) serology, which makes use of the interaction between a virus and antibody directed specifically against it; (iii) detection of viral nucleic acid.SamplingInjection in animal models (diseases), or eggsVirus culture in mosquito or mammalian cellsDetection of virus replicationCytopathic effect (syncitia, inclusion bodies, cell rounding, apoptosis, microarrays)HemadsorptionAntigen detection (immunocapture, Dot-blot, Western blot, Immunofluorescence, Immunohistochemistry, ELISAElectron microscopyGenome detection (Electrophoresis, in situ or membrane Hybridization, RT-PCR, ` multiplex or real time PCR, NASBA, Hybrid RNA-DNA, microarrays, Sequencing, substraction sequencing, random amplification-cloning-high throughput sequencing-blast)Reporter gene in genetically engineered cell linesSpecimens:Blood: serum, plasma, PBMC Swabs: Tracheal, nasopharyngeal, cutaneous… Fluids: CSF, urine, bronchoalveolar lavage, tracheal aspirate, tears…Tissues: Organs, skin… SamplingInjection in animal models (diseases), or eggsVirus culture in mosquito or mammalian cellsDetection of virus replicationCytopathic effect (syncitia, inclusion bodies, cell rounding, apoptosis, microarrays)HemadsorptionAntigen detection (immunocapture, Dot-blot, Western blot, Immunofluorescence, Immunohistochemistry, ELISAElectron microscopyGenome detection (Electrophoresis, in situ or membrane Hybridization, RT-PCR, ` multiplex or real time PCR, NASBA, Hybrid RNA-DNA, microarrays, Sequencing, substraction sequencing, random amplification-cloning-high throughput sequencing-blast)Reporter gene in genetically engineered cell linestracheal organ culturesSections through trachealo