HPLC法测定蒙药复方五味清浊中鞣花酸含量.docVIP

HPLC法测定蒙药复方五味清浊散中鞣花酸含量 　　【摘 要】 目的：建立蒙药复方五味清浊散中的鞣花酸含量测定方法。方法：采用HPLC方法，色谱柱为依利特BETASIL （250mm × 4.6mm，5μm），柱温40℃；流动相为乙腈-0.2%磷酸溶液（21∶79）；流速1mL/min；检测波长为254nm。结果：蒙药复方五味清浊散中鞣花酸在3.1625～202.4μg/mL范围内与峰面积程良好线性关系。回归方程为：Y=67818X-253716，R=0.9997。平均加样回收率为99.61%，RSD=2.37%。结论：该方法简单、准确、重复性好，可用于测定蒙药复方五味清浊散中鞣质的含量。 中国论文网 /1/viewhtm　　【关键词】 蒙药复方五味清浊散；鞣花酸；HPLC；含量测定 　　【中图分类号】R29 【文献标志码】 A 【文章编号】1007-8517（2017）03-0040-03 　　Mongolian Medicine Wuwei Qingzhuo Powders in the HPLC Method for Determination of Ellagic Acid Content 　　SU Duna1 CEli Muge2 HOU Yanhong3 TU Ya4 NIE Bo5* 　　1.Mongolian Medicine College of Pharmacy，Inner MongoliaUniversity for Nationalities，Tongliao 028000，China；2.Mongolian Medicine College of Pharmacy，Inner Mongolia Medical University，Huhehaote 010110，China；3.Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100027，China；4.Development Research Center of Traditional Chinese Medicine；China Academy of Chinese Medical Sciences，Beijing 100070，China；5.Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing，Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100070，China 　　Abstract：Objective To establish an HPLC quantitative method for the Ellagic acid in Mongolian medicine Wuwei Qingzhuo powders.Methods HPLC was used for Ellagic acid. The chromatography column was Elite BETASIL C18（250mm×4.6mm，5μm） and the column temperature was 40℃.The mobile phase was acetonitrile-0.2%phosphoric acid（21∶79）. The flow rate was 1mL/minand the detection wavelength was at 254nm.Results The regress equation for Ellagic acid was as the following： Y=67818X-253716（R=0.9997），and the linear range was 3.1625～202.4μg/mL.Theaverage recovery of Ellagic acid was 99.61%，RSD=2.37%. Conclusion This method is simple and convenient operation，is suitable for the Mongolian medicine Wuwei Qingzhuo powdersEllagic acid content determination. 　　Keywords：Mongolian Medicine Wuwei Qingzhuo Powders；Ellagicacid；HPLC；Content Determination 　　五味清浊散为蒙医临床常用药之一