蛇足石杉的孢子分析-特种植物育种与栽培专业论文.docxVIP

种后的污染率较小，无菌瓶甚至达到100%。无菌孢子在PDA培养基培养一段时间后未萌发，无吸胀且无菌丝出现。表明孢子壁不透水，孢子内也 无内生真菌的存在。(7)孢子的浸提液对2种作物种子萌发具有一定抑制作 用，相同浓度的浸提液对萝卜种子萌发的的抑制作用较强，不同溶剂提 取液对幼苗生根的影响也不同，而植株提取液对萝卜和绿豆种子的萌发 有不同程度的促进作用。(8)经过不同溶剂的处理，蛇足石杉的孢子壁出 现了一些变化，水的影响较小；水合氯醛可使孔穴清晰；浓盐酸和氢氧 化钠均使孢子变成黄色，且孔穴略模糊；而浓硫酸的影响却较小；间苯 三酚浓盐酸反应为负反应，未染成红色，木质化程度较低；而苏丹Ⅲ处 理的孢子壁为桔红色，表明孢子壁为脂质化。因此蛇足石杉的孢壁可耐 盐酸、硫酸等无机酸及冰醋酸等有机酸、氢氧化钠等无机碱、氯化钾等 无机盐、高温，不溶于无机酸、碱，略溶于生物透明剂等脂溶性溶剂。 结论：以上蛇足石杉孢子的特征可作为孢子鉴别及分类的依据；孢子 萌发困难的可能原因为：孢子壁较厚且不透水，孢子裂缝处内外壁均存 在且较厚，结合较紧密，破壁相对较难，内在物质释放较难；可能存在 有机酸等抑制物质存在等。关键词：蛇足石杉；孢子The spores study of Huperzia serrata AbstractObjective: Huperzine A is the active ingredient of Huperzia serrata which is currently the best treatment of Alzheimers disease drug, its propagation don’t make a major breakthrough now, these test will try to clarify the morphological characteristics of their spores and clarify possible reasons for germination barriers, to provide the reference for palynology and breeding by spores.Methods: Microscopic identification, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Ball Mills broken, conventional breeding techniques, tissue culture and seed germination experiments are applied.Results: The may results are as follows: (1) In the microscope, sporangia is reniform, composed of 2-4 layers of cells, the outermost cell wall is thickening and cork, scattered spore or a group of spores are fan-shaped, triangular or irregular, inner the spore wall nucleus and granule organelles can be seen. (2) Characteristics of SEM of the spores are these: spores of Huperzia serrata are tetrahedral, radial symmetry, there are three cracks on the white particles, the equatorial view is a half of a circular or fan-shaped, polarview is a blunt triangle, hole decoration, tetrad marks can clearly be seen. There are differences in Polar axis length, equatorial axis length, intensity of cavity, morphology of cracks and radiation zone. (3) Characteristics of TEM of the spores are these: Spores are fan-shaped or triangular, f