环境条件及六种农药对白僵菌菌株影响.docVIP

环境条件及六种农药对白僵菌菌株的影响 学校名称：内蒙古农业大学 作者姓名：朱营辉 摘要 研究了不同温度、紫外线及田间常用的2种杀虫剂、2种杀菌剂和2种除草剂对球孢白僵菌菌株的影响,结果表明:在17~26℃温度范围内,球孢白僵菌的菌落直径随着温度的升高而增大,在26℃条件下达到最大。经紫外线处理,该菌株抗紫外线能力很强,处理与对照菌落直径无差别;在常规浓度下，杀菌剂对球孢白僵菌的菌株生长完全抑制,而杀虫剂的抑制作用较弱,其中诺普信啶虫脒对球孢白僵菌菌株生长反而有促进作用,说明杀虫剂与球孢白僵菌相容性较好。所有除草剂在常规浓度下对菌株生长均有显著抑制作用，诺普信啶虫脒、布溜草和西玛津与球孢白僵菌的相容性相对较好,是菌药混用可能选择的对象。 关键词:球孢白僵菌；温度；紫外线；杀虫剂；杀菌剂；除草剂 Abstract This paper researched the influence of different temperatures,ultraviolet radiation, 2 pesticides、2 bactericide and 2 herbicide on Beauveria bassianamycelial growth·The results showed that the B·bassianacolony diameter increased with temperature increas-ing at range of 17~26℃.At 26℃, the clony diameter were the biggest. We had fungi which had strong anti-ultraviolet radiation ability through ultraviolet treatment.Under conventional concentration,there wasn’t difference between treatment and control colony diameters.The fungicides could inhibited the Acetamiprid growth completely.Whereas the insecticides had a slight inhibited effect on the mycelia growth and trichlorfon even accelerated the mycelia growth instead of inhibition.It showed that the insecticides were more compatible with B·bassiana.All herbicide significantly inhibited mycelium growth under conventional concentration.Acetamiprid, Glyphosate IPA Salt and Prometryne showed a comparatively higher compatibility with B.bassiana,providing IPM program good candidates of insecticides and herbicides for mixed use with fungal insecticides. Key words：Beauveria bassiana; temperature; ultraviolet radiation; pesticides; bactericide; herbicide 目 录 1引言 5 1.1白僵菌属的建立及白僵菌属种的分类历史 5 1.2 温湿度和紫外线对白僵菌的影响 6 1.2.1温度对白僵菌的影响 6 1.2.2湿度对白僵菌的影响 7 1.2.3紫外线对白僵菌的影响 8 1.3白僵菌的应用及研究进展 8 1.3.1白僵菌应用中存在的一些问题及对策 8 1.3.2白僵菌与化学农药混用的研究 9 1.3.3研究与生防应用前景展望 10 1.4目的及其意义 11 2材料和方法 12 2.1 实验材料 12 2.1.1实验器材 12 2.1.2PDA培养基 12 2.1.3供试菌株 12 2.1.4供试化学农药 12 2.2实验方法 12 2.2.1病原菌的分离纯化及其培养获得孢子粉 12 2.2.2孢子悬浮液的制备 13 2.2.3紫外线照射时间对球孢白僵菌菌株生长的影响 13 2.2.4