精品课件教案ppt 干细胞移植治疗ami临床研究进展.ppt

Circulation. 2010;121:2001-2011 Methods and Results: Vectors that encoded inducible suicide genes under the control of endothelium (endothelial nitric oxide synthase)-, smooth muscle (SM22α)-, and cardiomyocyte (α-MHC)-specific promoters, thereby allowing selective depletion of the individual cell lineage acquired by the transplanted undifferentiated bone marrow–derived cells. Depletion of eNOS-expressing cells (内皮细胞） was associated with a reduction of capillary and arteriole density and induced a deterioration of regional and global LVEF. The depletion of cells that expressed SM22-α（平滑肌细胞） induced a deterioration in contractile function. The elimination of cells that expressed the cardiac myocyte markerα-MHC（心肌细胞） did not significantly affect cardiac function. 移植途径 Intracoronary injection However, traditional reperfusion strategies fail to open the artery in some patients, making effective delivery impossible. The study demonstrated a safe and efficient approach to delivering bone marrow stem cells via a noninfarcted artery in an animal myocardial infarction model. Cardiovasc Ther. 2010 Mar 10. [Epub ahead of print] NOGA system 支持文献 Clinical implications The sample size of the REPAIR-AMI trial was not powered to definitely answer the question whether BMC administration is capable to modify mortality and morbidity after AMI. Therefore, this analysis should be viewed as hypothesis generating. As such, this analysis provides the rationale to design a larger clinical outcome trial addressing the clinical end. 精读（2） Patients from the Autologous Stem cell Transplantation in Acute Myocardial Infarction (ASTAMI) study were re-assessed 3 years after inclusion. Heart 2009 95: 1983-1989 Randomised, controlled trial Two university hospitals in Oslo, Norway The primary endpoint: The change in LVEF from baseline to 6 months measured by SPECT. Echocardiography and MRI were used for serial assessment of LV function. Secondary endpoints