蛋白翻译后修饰宣讲培训.pptx

第五讲 蛋白质翻译后修饰Post translational modificationHong XuE-mail: xuhong1685@163.comTel:(O)191352118 (QQ or 微信)各物种基因数量比较细菌：~4400酵母：~5800果蝇：~14000小鼠：~22000人：~25000PhenotypeGenotype橘生淮南则为橘，生于淮北则为枳，叶徒相似，其实味不同。所以然者何？水土异也！protein productsmRNAgenomic DNAfunctional proteintechnologylncRNAmicroRNA中心法则activity profilingdata integrationpost-translational modification analysisinferred activityemergingdata integrationbiological systemexpression profilingsubcellular localizationquantitative protein profilingprototypestructural determinationprotein linkage maps(dynamic)protein linkage maps(catalog)sequencingmatureOutlines（3W1H）What is Post-translational modification ?What types of Post-translational modifications are exist?What is the biological meanings of protein post-translational modifications?How to detect the Post-translational modification?What is Post-translational modification (PTM)?Protein modificationProtein modifications different types, including co-translational and post-translational inteinsCo- and Post-Translational Modifications Co-translational modificationsoccurs during protein synthesis N-terminal processing N-glycosylation Partial proteolysisPost-translational modificationsoccurs after protein synthesis phosphorylation methylation O-glycosylationPosttranslational modification (PTM) is the chemical modification or processing of a protein after its translation. It is one of the later steps in protein biosynthesis, and thus gene expression, for many proteins.Numerous （400 types）Diverse (organisms; proteins) Preference (e.g., phosphorylation：Ser Thr Tyr)What types of Post-translational modifications are exist?Posttranslational modifications including:Disulfide bond formationChemical modifications: Phosphorylation （1992 Nobel Prize） Acetylation Ubiquitination （2004 Nobel Prize） Glycosylation SUMOylation Methylation Succinylation ……Proteolytic processing……Posttranslational Modification Modification Charge-dependent change Acylation （酰化作用） loss of