(食品质量和 与安全研讨会)In vitro effects of anti-oxidants from fruits and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu.pptVIP

* In vitro effects of anti-oxidants from fruits and calyxes of Physalis pubescens L. on hepatocellular carcinoma cell line Malhavu Wang jingjing Liaoning medical university Laboratory studies have shown that various therapeutic effects are generally involved in plants of Physalis such as: antibacterial, anti-inflammatory, anticancer, immune adjustment and regulation of blood lipid level effects. objective This study aimed to identify whether, or not, antioxidants rich in phenols and flavonoids from fruits and calyxes of P. pubescens can be a candidate for the further development of an anti-hepatoma fraction, and if such biological effects coupled with reactive oxygen species (ROS) changes, can provide a direction for subsequent biological assay. Preparation of tested compounds Determination of flavonoid and phenolic content Cell culture Cell proliferation assay Induced-apoptotic property assay Measurement of ROS in Malhavu cells exposed to FTPF or CTPF Assessment of antioxidant activity of FTPF or CTPF My?presentation?will?cover?the?followingaspects: FTPF: total phenol and flavonoid from fruits CTPF: total phenol and flavonoid from calyxes Preparation of tested compounds CTPF CTPF Bing dried in oven at 50 °C for 24 h Powdered, stored at -20 °C 80% ethanol, at 60 °C, 3 h, ratio of sample-to-solvent : 1:70 (w/v) dissolved in DMSO solvents, filtrated with 0.22μm aseptic Millipore filter diluted with complete medium containing 10% FBS under aseptic conditions. stored at -20 °C until use Fig. 1. shows that the two tested compounds produced different biological effects on Mal cells. CTPF led to a significant dose and time-depend anti-proliferative property. However within the tested concentrations, FTPF presented an obvious promotion in the cell viability. Fig. 1. Effects of TPF from P. pubescens on cell viability. A: treated cells with FTPF. B: treated cells with CTPF. A1 and B1: cells are photographed under normal pattern. A2 and B2: cells are