cxcr4在卵巢癌转移中的作用及其机制分析word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 SLUG 和 Vimentin 的 mRNA 表达分别下降了 90.5%、26.1%、35.4%、93.5%、91.5% 和 78.3%，P0.05. 2. Western blot 结果显示特异性 CXCR4 shRNA 能够显著抑制 Wnt 经典通路中侵袭相 关基因 MMP-9 和 Vimentin 蛋白表达，P0.01。 结论 CXCR4 基因沉默能抑制 Wnt/?-catenin 通路主要相关基因的表达，CXCR4 可能通 过调控 Wnt/?-catenin 通路参与卵巢癌的侵袭转移。 关键词 CXCR4，Wnt/?-catenin 通路，卵巢癌 Effective Mechanisms of CXCR4 in Metastasis of Ovarian Cancer Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology M.D. Candidate : Jia Wang Supervisor : Prof. Zehua Wang Abstract Part One The Expression of CXCR4 in Ovarian Cancer and its Clinical Relation Objective To examine the expression of CXCR4 in malignant epithelial ovarian tumors and ovarian cancer cell lines, to investigate its relationship to clinicopathological features and the expression of β-catenin. Methods Immunohistochemical analysis was used to detect the expression of CXCR4 and β-catenin in 49 ovarian cancer tissues and 7 normal ovarian tissues and to analyze the relationship between CXCR4 and clinicopathological features in ovarian cancer. Pearson’s analysis was applied to study the correlation of CXCR4 and β-catenin expression. Real-Time PCR method was used to analyze the mRNA expression of CXCR4 in normal ovarian tissues, malignant epithelial ovarian tumors and ovarian cancer cell lines. Western blot were used to analyze the protein expression of CXCR4 in normal ovarian tissues, and ovarian cancer cell lines. Results Abnormal expression of CXCR4 and β-catenin were detected in ovarian cancer tissues. There was significant correlation between CXCR4 and β-catenin expression （r=0.628，P0.001）. The expression of CXCR4 was also correlated to the lymphatic metastasis（P=0.016）. The mRNA expression of CXCR4 was overexpressed by （6.10±2.4）、（6.01±0.3）、 （4.33±0.3）and（3.62±0.6）times in malignant ovarian tumors and ovarian cancer cell lines CAOV3、SKOV3 and A2780 respectively compared with normal ovarian tissue, the differenc