HPLC测定人尿液三苯双脒次级代谢物对苯二甲酸浓.docVIP

HPLC测定人尿液三苯双脒次级代谢物对苯二甲酸浓_基础医学论文 【摘要】 目的建立测定人尿液三苯双脒次级代谢物对苯二甲酸（tpa）浓度的高效液相色谱法（hplc）。方法 以阿魏酸为内标（internal standard, is），志愿者尿样稀释后经ultimate xbc18柱（4.6mm×200mm，5μm）、流动相甲醇－1%醋酸（35∶65，v/v）分离，在检测波长为240nm、流速为 0.8ml/min、柱温为30℃条件下采用hplc分析。结果在1～400μg/ml内，tpa与is的峰面积比值与浓度线性关系良好（r=0.9954），定量下限为1μg/ml，低、中、高浓度的相对回收率分别为（106.0±4.4）%、（107.4±4.2）%和（108.5±2.4）%，批内和批间相对标准偏差（rsd）小于8%。结论方法符合生物样品分析要求，可用于三苯双脒次级代谢物tpa尿液浓度的测定。【关键词】 三苯双脒 对苯二甲酸 高效液相色谱法 尿 terephthalic acid by hplc xu jie1,2, yuan guiyan1, wei chunmin1, wang benjie1, guo ruichen1 (1． institute of clinical pharmacology， qilu hospital of shandong university， jinan 250012， china; 2．department of bioengineering， zibo vocational institute， zibo 255013， shandong, china) to develop a hplc method for determination of tribendimidine metabolite terephthalic acid (tpa) concentration in human urine. methods diluted urine was separated on the ultimate xbc18 column (4.6mm×200mm, 5μm), eluted with methanol and 1% glacial acetic acid (35∶65, v/v) at 0.8ml/min and 30℃, and detected at 240nm with ferulic acid as an internal standard (is). results a good linearity was obtained from 1 to 400μg/ml (r=0.9954). the lower limit of quantitation (lloq) was 1μg/ml. the relative recoveries were (106.0±4.4)%, (107.4±4.2)% and (108.5±2.4)% at 3, 25 and 200μg/ml, and the intra and interbatch relative standard deviations (rsd) were less than 8% for all quality control samples. conclusion the developed hplc method is proved to meet the requirements of biological sample analyses, and is appropriate for determination of urinary tribenmidine metabolitetpa in human urine. key words: tribendimidine; terephthalic acid; high performance liquid chromatography; urine 三苯双脒（tribendimidine）是新型广谱驱肠虫药［13］，化学名为n,n′双［4′(1二甲氨基乙亚氨基)苯基］ 1,4苯二甲亚胺，po后于体内迅速分解为对（1二甲氨基乙亚氨基）苯胺（p(1dimethylamino ethylimino) aniline，dadt）和对苯二甲醛（1, 4phthaladehyde），对苯二甲醛经过体内酶氧化，迅速转化为对苯二甲酸（terephthalic acid，tpa）自尿中排泄；血中无对苯二甲醛检出。wwW.133229.cOm国内外文献多报道三苯双脒临床应用或药效学，鲜有其人体内处置过程［4］。文献报道［56］人尿液对苯二甲