CTGF对肝癌细胞生物学行为的影响及其与PPARγ之.docVIP

CTGF对肝癌细胞生物学行为的影响及其与PPARγ之_肿瘤学论文 【摘要】 目的 探讨结缔组织生长因子（ctgf/ccn2）对肝癌细胞生物学行为的调控作用，及其与pparγ在肝癌中的相互关系。方法 免疫组织化学法检测hepg2细胞中ctgf及pparγ蛋白的表达。经不同浓度、不同时间的ctgf抗体封闭处理人肝癌细胞hepg2后,mtt法测定细胞活力,博依登小室测定细胞侵袭及迁移特性的改变。经pparγ激动剂(rosiglitazone,罗格列酮)处理hepg2细胞后， rtpcr 检测ctgf mrna表达的改变。结果 hepg2细胞表达ctgf及pparγ蛋白。肝癌细胞经ctgf抗体处理后，其增殖受抑制,且呈时间和剂量依赖性,同时细胞的侵袭、迁移能力也受到抑制。hepg2 细胞经罗格列酮处理后，其ctgf mrna的表达下降。结论 ctgf可以调控肝癌细胞的增殖、侵袭及迁移能力。pparγ调控肝癌细胞的生长，部分是通过改变ctgf的表达来实现的。 【关键词】 ctgf; pparγ; 侵袭; 迁移; 人肝癌细胞influence of ctgf on hepatocellular carcinoma cells and correlation with pparγ cai wei, zhang fang, liu wei, yang aijun, wang chenyu, li min institute of pathology, lanzhou university, lanzhou 730000, china corresponding author: li min, email: limin0606@163.comabstract：objective to investigate the effect of connective tissue growth factor (ctgf/ccn2) on the growth of human hepatocellular carcinoma cells, and explore the correlation between pparγ and ctgf. methods the expression of ctgf and pparγ protein in hepg2 cell was detected by immunohistochemistry.hepg2 cells treated with the antibody of ctgf, at different concentrations for different periods. the proliferation of hepg2 cells was assessed by mtt. the change of invasion and immigration of hepg2 cells were detected by boyden. the mrna level of ctgf in hepg2 cells treated with rosiglitazone was detected by rtpcr. results hepg2 cells express ctgf and pparγ protein. mtt assay demonstrated that antibody of ctgf had inhibitory effect on the proliferation of hepg2 cells in a timeand dosedependent manner.the invasion and immigration of hepg2 cells were inhibited,too. the result of rtpcr demonstrated that the mrna level of ctgf was downregulated after treated with rosiglitazone. conclusion the antibody of ctgf could inhibit the proliferation, invasion and immigration of hepg2 cells. the mechanism of pparγ regulating the growth of hcc cell may be associated with downregulating the expression of ctgf. key words：ctgf; pparγ; invasion