广州老年人流感血清抗体水平监测.docVIP

广东地区2012-2013年pH1N1流感病毒血凝素基因进化及变异特征 黄钟洲1，2，梁丽君1，黄平1，邹丽容1 ( 511430 广州，广东省疾病预防控制中心广东省应急病原学检测重点实验室1；510515 广州，南方医科大学第二临床医学院2) [摘要]　目的　了解广东地区2012-2013年新型H1N1(pH1N1)病毒血凝素(HA)基因、表位和受体结合位点(RBS)进化和变异特征。 方法　检测广东地区2012-2013年分离的pH1N1毒株HA基因核苷酸序列，与GenBank中全球相应毒株序列比对，应用MEGA 6.05构建进化树，通过Chimera v1.8.1建模并标记，应用DATAMONKEY筛选HA选择性位点。结果　与疫苗株A/California/07/2009的HA基因比较，广东地区2012-2013年pH1N1毒株的HA基因同源性为98.1%~98.7%；变异位点包括S160G(抗原表位)和R222K(RBS)，正向选择位点为S202T位点。A/Guangdong /64/2013毒株发生V6I、N55S、S101G、A151V、H155Q、S160G和A214T变异。结论　广东2012-2013年pH1N1毒株HA基因变异导致了HA抗原表位和RBS的变异；毒株A/Guangdong/ 64/2013发生了包括抗原表位在内的7个位点的置换，这可能是下个流感流行季节优势毒株的分子基础。 [关键词]　pH1N1；血凝素；抗原表位；进化；变异 [中图法分类号] R373;Q933　 [文献标识码] A Molecular evolution and variation of pH1N1 virus hemagglutinin genes in Guangdong，2012-2013 Zhongzhou Huang1，2，Lijun Liang1，Ping Huang1，Lirong Zou．（1Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430；2The second Clinical College, Southern Medical University, Guangzhou 510515, China) [Abstract]　Objective　To reveal characteristics and variability of hemagglutinin(HA) genes of pandemic H1N1 (pH1N1) viruses isolated from Guangdong during 2012-2013．Methods　The HA genes of Guangdong influenza pH1N1 viruses isolated during 2012-2013 were sequenced and compared with global HA genes downloaded from GenBank．The phylogenetic tree and the molecular model of HA genes was conducted respectively by MEGA 6.05 and Chimera v1.8.1 as the selected sites were analyzed by DATAMONKEY．Results　Compared with HA of vaccine strain A/California/ 07/2009，the HA genetic homologies of Guangdong strains during 2012-2013 reached to 98.1%~98.7%．There were some amino acid substitutions including S160G (Sa epitope) and R222K (RBS)．Residue S202T was revealed to be the positively selected site．The HA amino acid mutations of A/Guangdong/64/2013 occurred in V6I, N55S, S101G, A151V, H155Q, S160G and A214T．Conclusion　The mutations in HA genes of Guangdong pH1N1 viruses during 2012-2013 leaded to variations in antigenic e