stable inhibition of mmu-mir-466h-5p improves apoptosis resistance and of protein production in cho cells文档.pdfVIP

NIH Public Access Author Manuscript Metab Eng . Author manuscript; available in PMC 2014 March 01. N Published in final edited form as: I H Metab Eng . 2013 March ; 16: 87–94. doi:10.1016/j.ymben.2012.12.004. - P A A u Stable inhibition of mmu-miR-466h-5p improves apoptosis t h o resistance and protein production in CHO cells r M a a,b a b a,* n Aliaksandr Druz , Young–Jin Son , Michael Betenbaugh , and Joseph Shiloach u a s Biotechnology Core Laboratory NIDDK, NIH Bldg 14A Bethesda, Maryland 20892 c r i b p Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore t Maryland 21218 Abstract MiRNAs have been shown to be involved in regulation of multiple cellular processes including apoptosis. Since a single miRNA can affect the expression of several genes, the utilization of miRNAs for apoptosis engineering in mammalian cells can be more efficient than the N I conventional approach of manipulating a single gene. Mmu-miR-466h-5p was previously shown H - to have a pro-apoptotic role in CHO cells by reducing the expression of several anti-apoptotic P A genes and its transient inhibition delayed both the activation of Caspase-3/7 and the loss of cell A viability. The present study evaluates the effect of stable inhibition of mmu-miR-466h-5p in CHO u t cells on their ability to resist apoptosis onset and their production properties. The expression of h o mmu-miR-4