麋鹿粪便中果胶降解菌紫外诱变研究.docVIP

盐 城 师 范 学 院 毕业论文 2013－2014学年度 麋鹿粪便中果胶降解菌 的紫外诱变研究 学生姓名 王 波 学 院 生命科学与技术学院 专 业 生物工程 班 级 10（4） 学 号 指导教师 王欢莉 2014年 月 日 麋鹿粪便中果胶降解菌紫外诱变的研究 摘 要 果胶是植物中的一种酸性多糖物质主要存在于植物的细胞壁和细胞内层，为内部细胞的支撑物质。随着对果胶及果胶酶研究的日益深入， 其应用领域已逐渐拓宽。尽管如此，就果胶酶的高效、多功能特点而言，其应用尚有较大的发展空间。本文以国家一级保护动物——麋鹿粪便为材料，通过初筛和复筛得到一株果胶降解菌A并对其进行紫外诱变；设置4因素3水平对进行诱变，得到一株编号为WB8-1的菌株其酶活8165.2 U，比出发菌株提高了32.8%。得出最佳诱变条件为紫外光照时间为120 s、紫外光照距离为30 cm、菌浓为稀释10-5，菌龄为4。 【关键词】 麋鹿粪便 ；果胶降解菌；紫外诱变；酶 study of Pectin-Degrading Strains by UV mutagenesis in Elaphurus davidianus’s feces Abstract Pectin is a kind of acidic polysaccharides of plant material mainly exist in the plant cell wall and cell lining, support material for internal cells. With the increasingly deepening of research on pectin and pectinase, its application field has gradually widened. However, in terms of high efficiency, multifunctional characteristics of pectinase, its application has larger development space. In this paper the Elaphurus davidianus’s feces was used as the experimental materials which was a kind of national level to protect animals .Through primary screening test and secondary screening the pectin-Degrading Strain-A was got for UV mutagenesis .In this study the experiment was designed Set 4 factors 3 level to mutagenesis the pectin-Degrading Strains ; a strain named WB 8-1that the enzyme activity about pectin-Degrading is 8165.2 U was got , which was increase 32.8% over the starting strain.It was concluded that the best Mutation conditions were UV lighting time -120 s, UV light distance -30 cm, the best concentration of the bacteria-10-5 , and bacteria’s age- 4 days. 【 key words 】 Elaphurus davidianus’s feces; Pectin-Degrading Strains; Ultraviolet mutagenesis; pectinase 目 录 1.引 言 4 1.1.果胶的简要介绍 4 1.1.1.结构与生理功能 4 1.2.果胶酶的种类 5 1.3.实验菌种的可研究性 5 2.材料与方法 6 2.1.材料 6 2.1.1.菌种采集 6 2.1.2.培养基种类及作用 6 2.1.3.实验器材 6 2.1.4.实验