先兆子痫患者血清血管紧张素II1型受体自身抗体.docVIP

先兆子痫患者血清血管紧张素II 1型受体自身抗体对大鼠主动脉血管平滑肌细胞内钙水平的影响 曾庆源 肖宏2 朱峰3（430060武汉,武汉市第三医院1，武汉市第一医院2，430022武汉,华中科技大学同济医学院附属协和医院心内科、心血管病研究所3） [摘要] 目的 探讨先兆子痫患者血清中存在的血管紧张素II 1型（AT1）受体自身抗体对大鼠主动脉血管平滑肌细胞（VSMC）增殖的作用，并探索其作用的信号分子机制。方法 以AT1受体的胞外第二环作为抗原，亲和层析法提纯先兆子痫患者AT1受体自身抗体。应用AT1受体自身抗体刺激培养VSMC，钙离子荧光探针Fluo-3/AM负载VSMC，共聚焦显微镜检测VSMC细胞质游离钙浓度，观察抗体对VSMC胞浆游离钙水平的影响。结果 与抗体阴性血清相比，以AT1受体自身抗体刺激的VSMC胞内游离钙水平增高，并呈现剂量－效应依赖关系，AT1受体特异性拮抗剂氯沙坦与AT1受体胞外第二环抗原表位肽段（A-F-H-Y-E-S-Q）均能抑制该效应，其促进VSMC胞内游离钙水平增高的效应与血管紧张素II（Ang II）类似。结论 先兆子痫患者血清AT1受体的自身抗体与Ang II一样能够特异性与升高VSMC胞内钙浓度，在先兆子痫患者的血压升高及血管炎症中可能发挥作用。 [关键词]先兆子痫；血管紧张素II 1受体；细胞增殖；信号分子机制 [中图法分类号]　R322.12；R543. 5；R966 [文献标志码]　A Autoantibodies against the AT1 receptor from patients with preeclampsia induce the increase of intracellular Ca2+ mobilization of cultured rat vascular smooth muscle cells Zeng Qingyuan1, Xiao Hong2, ZhuFeng3 (1Wuhan Third Hospital, Wuhan 430060; 2Wuhan First Hospital; 3Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China) [Abstract] Objective The autoantibodies against the AT1 receptor autoantibodies were described in the patients with preeclampsia. This study will explore the autoantibodies against the AT1 receptor in patients with preeclampsia, and will investigate the ability of these antibodies to induce the proliferation of cultured rat vascular smooth muscles cells (VSMC). Methods The serum of each patient with preeclampsia was detected for the presence of the autoantibodies by ELISA. The autoantibodies were purified by an affinity-column and used to stimulate the cultured rat VSMC. Intracellular Ca2+ mobilization in VSMC was measured using a calcium fluorescent indicator Fluo-3/AM. Results The autoantibodies could induce an increase of the intracellular Ca2+ mobilization in a dose-dependent manner. Both losartan and a 7-amino-acid peptide (A-F-H-Y-E-S-Q) corresponding to a portion of second extracelluar loop of the AT1 receptor could abolish the effec