使用微孔板光谱分析仪和平板计数测定阪崎肠杆菌的生长.pdfVIP

※生物工程 食品科学 2008, Vol. 29, No. 07 281 袁 飞，徐宝梁，陈 颖，赵贵明，黄文胜，吴亚君，张菲菲，赵勇胜，杨海荣，王 晶 ( 中国检验检疫科学研究院食品安全所，北京 100025) ：本实验采用96 孔板培养阪崎肠杆菌，然后由微孔板实时监测阪崎肠杆菌的生长。采用微孔板光谱分析 仪和传统的平板方法测定阪崎肠杆菌数量之间具有非常好的相关性(R2=0.92) ，且可缩短测定时间( 由至少24h ，缩短 为小于10h)。阪崎肠杆菌菌株36 ℃培养末期数量相近，但生长曲线不同。进一步研究17 株阪崎肠杆菌菌株42 ℃ 生长情况，发现微孔板光谱分析仪方法不仅能快速简便测定生长，且可以一种实时的方式知道阪崎肠杆菌在整个培 养过程中的生长情况。研究结果表明，采用该方法可将阪崎肠杆菌菌株根据生长曲线的差异进行表型分类，该表 型与阪崎肠杆菌菌株生化反应表型结果一致。 ：阪崎肠杆菌；微孔板光谱分析仪；平板计数 Growth of Enterobacter sakazakii Monitored by Microplate Luminometer YUAN Fei ，XU Bao-liang ，CHEN Ying ，ZHAO Gui-ming ，HUANG Wen-sheng ，WU Ya-jun ， ZHANG Fei-fei ， ZHAO Yong-sheng ，YANG Hai-rong ，WANG Jing (Food Safety Institute, Chinese Academy of Inspection and Quarantine, Beijing 100025, China) Abstract ：Experiments were undertaken to determine the growth of E. sakazakii under different temperatures by microplate luminometer. 16 Strains of 17 E. sakazakii strains (including ATCC 29544) used in this study were isolated from dried infant milk formula powder on China retail market. The results showed that the E. sakazakii counts determined by microplate luminometer are perfectly correlated with those determined by conventional plate counting method (r=0.92). E. sakazakii in brain heart infusion broth can be quickly monitored with much shorter time (no more than 10 h) than that of conventional plating method (at least 24 h). Although at the end of incubation the amounts of E. sakazakii strains are close under 36 ℃, but the growth curves are different. And the further research results of 17 E. sakazakii strains being incubated at 42 ℃ indicated that the microplate luminometer method provides a convenient, rapid and real-time way to monitor the growth of E. sakazakii . The r