冀鲁豫棉区Bt棉Cry1A蛋白表达及对棉铃虫控制效果监测课件.pdfVIP

花 学 报 Cotton Science 20 13 25 渊5 冤院459~466 鲁豫棉区Bt 棉Cry 1A 蛋白表达及对棉铃虫 控制效果监测 丽敏 雒珺瑜 刘全义 杨子山 张 帅 王春义 崔金杰* 棉花生物学国家重点实验室/ 中国农业科学院棉花研究所袁河南安阳455000冤 摘要院2008 2012 年连续5 年对冀鲁豫三省抗虫 田进行田间系统监测尧室内抗虫性生物测定和Bt 外源杀虫 蛋白表达量检测袁共涉及44 个植 县区129 个抗虫棉品种遥 田间监测结果表明院2011 年监测棉田2 代棉铃虫 发生期田间百株残虫量为20 头袁达到了防治指标 13 头冤袁其他年份2 代 铃虫均低于防治指标曰顶尖被害率 和蕾铃被害率均较低遥 室内生物测定结果表明院2 代 铃虫 正死亡率呈现先升高后降低的趋势袁3 代和4 代 铃虫校正死亡率整体呈现下降的趋势遥 外源杀虫蛋白含量测定结果表明院苗期叶片外源杀虫蛋白含量呈现 逐年下降的趋势袁蕾期和花铃期叶片外源杀虫蛋白略有上升袁但均低于同年苗期叶片外源杀虫蛋白含量遥 关键词院抗虫 曰抗性监测曰种群动态 中图分类号院S435.622 文献标志码院A 文章编号院1002-7807 渊20 13冤05-0459-08 LYU Li-min, LUO Jun-yu, LIU Quan-yi, YANG Zi-shan, ZHANG Shuai, WANG Chun-yi, CUI Jin-jie * (S 455000, ) From 2008 to 20 12, using the methods of field monitoring, bioassay, expression of Bt exogenous proteins, we detected 129 Bt cotton varieties at 44 plant cotton sections in Hebei, Shandong and Henan cotton region. The field monitoring revealed that No. of remnant cotton bollworm per 100 plants was 20 for the second generation cotton bollworm in 2011, over the control index, but that of other years lower than the control index; the rate of injured cotton stem-tips, buds and bolls were at a low lev- el. The results of the bioassay showed that the corrected mortality for the second generation bollworm increased at first but then decreased, and that of the third and fourth generation bollworm showed a decreasing tendency. The Bt exogenous protein assays showed expression was decreasing year by year at the seedling stage, whereas the expression of Bt exogenous protein in the bud- ding and bolling stage were increasing a little, but lower than those in seedling stage. Bt cotton; resistance monitoring; population dynamics 20 世纪90 年代 棉铃虫在我国主要棉区大 铃虫对Bt 杀虫蛋白的抗性已经