[医药卫生]组织工程支架材料20110509.pptVIP

以硫酸肝素复合胶原蛋白并结合特定条件下的冷冻干燥技术制备内部结构具有纵行的、平行排列的微管材料使其在三维结构上高度仿生神经。 Peptide-functionalized PLL-g-PEG PLL(20)-g[3.5]-PEG(2)/PEGbiotin(3.4)50% - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + (strept)avidin biotin Immobilization through (strept)avidin-biotin Immobilization through other specific interactions Nitrilotriacetic acid (NTA) 生物材料的表面修饰： * 表面修饰的方法 * 生物大分子固定方法 * 抗非特异性吸附的表面 * 促进细胞特异性黏附的表面 * 生物材料表面图案化 Protein-resistant surfaces (bioinert or non-fouling surfaces) Three substances that are widely used to effectively reduce or avoid protein adsorption and cellular attachment are poly(ethylene glycol), phosphorylcholine, and polysaccharides. * 抗非特异性吸附的表面 Figure . (a) Molecular Structure of PLL-g-PEG. By variation of j, k and m the architecture of PLL-g-PEG can be adjusted to any needs [9] (b) Electrostatically adsorbed PLL-g-PEG on TiO2 at neutral pH via the negatively charged metal oxide surface and positively charged amino groups of the PLL side chains Figure. Schematic representation of some PEG chain structures at the interface. Non-fouling properties are expected from “interacting mushroom” and “brush-like” conformation. Their dense structure should provide an interfacial barrier to prevent the proteins from interacting with the underlying substrate. Based on poly(ethylene glycol) Hydrogen Bonding Unique water solubility Steric repulsion (density and chain length) Hydrogen-bonding networks PEG derivatives Schematic representations of alkylating PEGs Schematic representations of acylating PEGs Schematic representations of thiol-reactive PEGs Schematic representations of PEG-hydrazide Fig. Structural analogy between DPPC (a) and MPC:alkyl methacrylate (1:2) copolymers (b). 2-Methacryloyloxyethylphosphorylcholine (MPC). The phosphorylcholine headgroup. Based on phosphorylcholine Fig. 13. Cross-sections through (a) and (b) uncoated and (c) and (d) PC-coated ureteric stents. Immobilization by spin coating, LB deposition, adsor