微波消解-氢化物发生-原子荧光法测定中药中的砷-中国药品标准.PDFVIP

2005 6 2 ( 70) Drug St nd rds of Chin 2005,vol. 6 N o. 2 , [ 1] ( ) . W S-338( X-294) -2000( 1) [2] . 1995 : 6 -- ( 264000) 阮　建 　刘　群 　郝志巧 : 建立了微波消解-氢化物发生-原子荧光法测定中草药中砷含 的方法。: 利用微波消解仪对中草药进行前处 理, 以盐酸为测定介质, 硫脲-抗坏血酸为预还原剂, 硼氢化钾为还原剂, 用氢化物发生-原子荧光法测定砷含 。: 砷含 在 4 ～100ng ·ml- 1浓度范围内线性关系良好( r = 0. 9998) , 方法的检出限为0. 22ng ·ml- 1 , 相对标准偏差( RSD) 为 1. 3% ～ 5. 1% , 回收率为94. 1% ～101. 3% 。: 本方法简便, 快速, 灵敏, 准确, 可用于中草药中砷的测定。 微波消解; 氢化物发生; 原子荧光法; 中草药; 砷 - Determination of Arsenic in Chinese Herbs by Microwave Digestion Hydride Generation Atomic Fluorescence Spectrometry Ru n Ji n Liu Qun H o Zhiqi o ( Yantai I nst it utef or D rug Cont rol S handong Pr ov inci al Yantai L u Y in P harmaceu tical L imi ted Comp any , Y antai 264000) Abstract Objective: T o est blish method for the determin tion of rsenic in Chinese herbs. Methods: The s mples were tre ted by microw ve digestion. In test , hydrochloric cid w s the medium, t hioure - scorbic cid w s the pro-reducing gent , . - pot ssium borohydride w s t he reducing gent T he contents of rsenic w ere determined by hydride gener tion tomic fluores- cence spectrometry. esults: W ithin 4100ng ml- 1, rsenic h d good line rity ( r = 0. 9998) . The detection lim it of the method w s 0. 22ng . ml- 1, the rel tive st nd rd devi tion( RSD) w s in t he r nge of 1. 3% 5. 1% , nd the recovery w s in 94. 1% 10 1. 3% . : , , , the r nge of Conclusion This method is simple r pid sensitive nd ccur te nd it c n be used t o determine rsenic in Chinese herbs. Key words: Microw ve digestion; hydride gener tion; t omic fluorescence; Chinese herbs; rsenic