离子色谱法测定大蒜多糖的单糖组成和含量-ThermoFisherScientific.PDFVIP

·74· Chinese Journal of Information on TCM Oct.2014 Vol.21 No.10 离子色谱法测定大蒜多糖的单糖组成和含量 1 2 1 1 1 柏冬 ,韩春霞 ,王瑞海 ,刁作彬 ,刘丽梅 1.中国中医科学院中医基础理论研究所,北京 100700；2.赛默飞世尔科技(中国)有限公司，北京 100007 摘要：目的 建立测定大蒜多糖的单糖组成和含量的离子色谱法。方法 以氨基酸分离柱,氢氧化钠/醋酸 钠溶液梯度洗脱,脉冲积分安培检测器 (Au为工作电极,Ag/AgCl 为参比电极)检测,测定大蒜多糖水解产生的单 糖组成及含量。结果 大蒜多糖主要由阿拉伯糖、半乳糖、葡萄糖、木糖、果糖等单糖组成,各单糖基本达到 分离,线性关系良好,相关系数均＞0.998,平均回收率均在 95%～105%之间。结论 所建立的方法准确、简单, 可用于大蒜多糖中单糖的含量测定和质量控制。 关键词：离子色谱法；大蒜多糖；单糖；半乳糖；葡萄糖；甘露醇；果糖 DOI：10.3969/j.issn.1005-5304.2014.10.022 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2014)10-0074-03 Determination of Composition and Content of Monoses in Garlic Polysaccharide by Ion Chromatography 1 2 1 1 1 BAI Dong , HAN Chun-xia , WANG Rui-hai , DIAO Zuo-bin , LIU Li-mei (1.Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China ；2.Thermo Fisher Scientific, Beijing 100007, China) Abstract ：Objective To establish a method to analyze the composition and content of monoses in the garlic polysaccharide by ion chromatography. Methods The monoses in the solution of garlic polysaccharide hydrolysised by trifluoroacetic acid were determined by amino PAC column, eluted by gradient hydroxide sodium and identified by ampere detector (AU as working electrode, Ag/AgCl as reference electrode). Results The garlic polysaccharide was mainly composed by arabinose, galactose, glucose, xylose and fructose. Good resolution could be achieved among the above mentioned five kinds of monoses. Their linear correlations over the investigated concentration were above 0.998, while the average recovery rates were in the range of 95%-105%. Con