几种芳香酚室内抑菌活性测定毕业论文.docVIP

河 南 科 技 大 学 毕 业 设 计（论 文） 几种芳香酚的室内抑菌活性测定 姓 名 翟贝贝 院 （系） 林学院 专 业 植物保护 指导教师 车志平 2015年6月8日 几种芳香酚的室内抑菌活性测定 摘 要 近年来，由于番茄灰霉病和烟草枯萎病的危害呈逐年上升趋势，对其防治方面的研究也随之增多。另外，由于杀菌剂的大量使用导致病害抗药性问题严重加剧，发现新的药剂显得尤为重要。为了筛选出较理想的替代药剂，本研究采用菌丝生长速率法测定了几种芳香酚类化合物对番茄灰霉病和烟草枯萎病的室内抑菌活性。实验结果显示，1-萘酚、麦芽酚、芝麻酚、奎宁、丹皮酚和阳性对照恶霉灵对于番茄灰霉病的EC50值分别为0.0179 mg/mL，0.0913 mg/mL，1.0232 mg/mL，0.1520 mg/mL，0.0737 mg/mL和0.0380 mg/mL；1-萘酚、芝麻酚、奎宁、丹皮酚和阳性对照恶霉灵对于烟草枯萎病的EC50值分别为0.0320 mg/mL，0.1182 mg/mL，1.5070 mg/mL，0.1891 mg/mL，0.0743 mg/mL。麦芽酚对于烟草枯萎病具有促进作用，与本实验研究方向相悖。结果表明，1-萘酚对番茄灰霉病和烟草枯萎病均有较好的抑制作用，其抑菌活性超过了已商品化药剂恶霉灵。本研究初步筛选出防治番茄灰霉病和烟草枯萎病较好的芳香酚类化合物，为今后开发该类新型杀菌剂提供了理论依据。 番茄灰霉病；烟草枯萎病；芳香酚；；生长速率法 THE INDOOR ANTIBACTERIAL ACTIVICIYY OF SEVERAL KINDS AROMATIC PHENOL ABSTRAC In recent years, as a result of tomato grey mould and the harm of tobacco to blight showed a trend of rising year by year, the study of its prevention and control has said. In addition, due to large amounts of fungicide use lead to serious problems in plant disease resistance, it is particularly important to discover new drug. In order to select ideal alternative agents, in this paper, the mycelium growth rate method is adopted to determine several aromatic phenolic compounds of indoor bacteriostatic activity of tomato grey mould and tobacco blight. The experimental results showed that 1-naphthol, maltol, sesame phenol, quinine, mildew and positive controls are evil for tomato grey mould. The values of effective concentration (EC50) were 0.0179 mg/mL, 0.0913 mg/mL, 1.0232 mg/mL, 0.1520 mg/mL, 0.0737 mg/mL and 0.0380 mg/mL; 1-naphthol, sesame phenol, quinine, mildew and positive controls are also evil blight for tobacco. The values of EC50 were 0.0320 mg/mL, 0.1182 mg/mL, 1.5070 mg/mL, 0.1891 mg/mL, 0.0743 mg/mL. Maltol has promoting effect on tobacco blight, contrary to this experiment research direction. Results show that 1-naphthol for tomato grey mould and tobacco blight all have better inhibition and effect than the commercialization of mil