抗生素与生物固体耐药基因（英文PPT）Antibiotics and Resistance Genes in BiosolidsPPT.pptVIP

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抗生素与生物固体耐药基因（英文PPT）Antibiotics and Resistance Genes in BiosolidsPPT.ppt

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抗生素与生物固体耐药基因（英文PPT）Antibiotics and Resistance Genes in BiosolidsPPT

* Antibiotics and Resistance Genes in Biosolids Mary Stromberger Soil Microbiology Department of Soil and Crop Sciences What are Biosolids? Organic, treated solids and semi-solids recovered during wastewater treatment Treated to reduce pathogen and heavy metal loads Suitable for soil amendment Source of organic matter, plant nutrients, but also heavy metals and salts Recycling of Biosolids via land application: Emerging concerns Dioxin? Endocrine disruptors? Nonylphenols Flame retardants Denver Post 10/5/2004 Pharmaceuticals? Prescription drugs Antibiotics Therapeutic agents as emerging contaminants in biosolids: persistence and impact on soil microbial communities 2-year project funded by EPA Region 8 Objectives: Screen biosolids collected from different wastewater treatment facilities for kinds and amounts of antibiotics present Determine the persistence of selected antibiotics, applied alone or along with biosolids, in soils under aerobic conditions Determine the effects of selected antibiotics on microbial activity and microbial antibiotic resistance patterns in soil during aerobic incubation Determine the long-term effects of biosolids, land applied to field soils, on antibiotic resistance patterns of soil microbial communities. Are there antibiotics in biosolids? erythromycin tetracycline Concentration of sulfonamides in biosolids (μg kg-1) Anaerobic A Anaerobic B Anaerobic C Aerobic A Aerobic B Aerobic C STZ 7.1 10.7 7.0 5.6 5.3 BDL SMR BDL BDL 6.8 6.5 BDL 2.6 SMT 5.2 BDL BDL BDL BDL 4.6 SCP 10.6 6.6 6.2 6.9 3.8 3.2 SMX 9.2 BDL BDL 9.5 5.1 7.1 SDM 3.2 BDL 3.4 9.9 2.2 5.4 STZ: Sulfathiazole, SMR: Sulfamerazine, SMT: Sulfamethazine, SCP: Sulfachlorpyridazine, SMX: Sulfamethoxazole, SDM: Sulfadimethoxine BDL: Below detection limit Concentration of tetracyclines in biosolids (μg kg-1) Anaerobic A Anaerobic B Anaerobic C Aerobic A Aerobic B Aerobic C TC 843.3 1147.2 1986.6 285.1 1345.6 142.3 OTC 10671.3 386.2 1413.7 6787.0 324.3 26.1 CTC 53.8 BDL B