钩吻素子在人源肠Caco-2细胞单层模型中的吸收转运研究.docVIP

精品论文 参考文献 钩吻素子在人源肠Caco-2细胞单层模型中的吸收转运研究 张华 吴水生 于虹敏 　　（福建中医药大学 福建 福州 350122） 　　【摘要】 目的：研究马钱科胡蔓藤属植物钩吻种钩吻素子在人源肠Caco-2 细胞单层模型中的吸收特性。方法：利用人源结肠腺癌细胞Caco-2 细胞单层模型测试钩吻素子从AP端到BL端以及BL端AP端2个方向的转运过程研究，采用HPLC法对钩吻素子进行定量分析，计算累积吸收量和表观渗透系数(Papp)，并与对照药普萘洛尔和阿替洛尔进行比较。结果：不同浓度钩吻素子由AP端到BL端 (A-B面)的Papp、吸收量与浓度成线性关系，而由BL端到AP 端(B-A面) 的Papp约为A-B面的2倍，提示存在外排现象。结论：钩吻素子可能以被动扩散为主要转运方式被小肠上皮细胞摄取和转运，可以通过小肠上皮细胞被动吸收进入体内。 　　【关键词】 钩吻素子；Caco-2细胞模型；吸收；转运 　　【中图分类号】R28 【文献标识码】A 【文章编号】2095-1752（2016）14-0056-03 　　 　　Koumine element under anthropogenic optimization intestinal Caco - 2 the absorption of cell monolayer model transfer research 　　Zhang Hua, Wu shuisheng *, Yu Hongmin. Fujian University of Traditional Chinese Medicine, Fujian Province, Fuzhou 350122, China 　　【Abstract】 Objective Study plants of the genus loganiaceae hu vines clasps to kiss a kiss element optimization in anthropogenic bowel Caco - 2 cells the absorption characteristics of single layer model. Methods Using optimization anthropogenic colonic cancer cells Caco - 2 cell monolayer model, test Koumine from the AP to the BL, and BL end AP end, the direction of the two transfer process. Of the hook kiss element child by HPLC method, quantitative analysis, calculating the cumulative absorption and permeability coefficient (Papp), and with propranolol and atenolol. Results Koumine different concentrations by AP end to BL end (A - B side) of Papp, absorbed dose and A linear relationship with concentration, and by the BL end to AP end (B - A side) of Papp is about 2 times A - B below, prompt outside phenomenon exists. Conclusions; Koumine by passive diffusion as the main transportation way, by small intestinal epithelial cells to absorb and transport, can passive absorption into the body through the intestinal epithelial cells. 　　【Key words】 Koumine; Caco - 2 cell model; Absorption; Transshipment 　　钩吻（Gelsemium elegans Benth.）为马钱科胡蔓藤属植物，又名胡蔓藤、断肠草、毒根、大茶药、野葛等,是世界著名的剧毒植物。药用其根、茎、叶。性温味辛、苦，有大毒[1]。通常只作外用,具有祛风散