Screening and Quantitative Analysis for Sulfonylurea-Type Oral….pdfVIP

Journal of Health Science, 51(4) 453–460 (2005) 453 Screening and Quantitative Analysis for Sulfonylurea-Type Oral Antidiabetic Agents in Adulterated Health Food Using Thin-Layer Chromatography and High-Performance Liquid Chromatography *, a, b a b, c a Kenichi Kumasaka, Takashi Kojima, Hideo Honda, and Kayo Doi a Chemistry Division, Kanagawa Prefectural Institute of Public Health, 1–3–1, Shimomachiya, Chigasaki, Kanagawa 253–0087, Ja- b pan, Second Department of Physiology, School of Medicine, Showa University, 1–5–8, Hatanodai, Shinagawa-ku, Tokyo 142–8555, Japan, and cDepartment of Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432–1, Horinouchi, Hachioji, Tokyo 192–0392, Japan (Received February 23, 2005; Accepted March 23, 2005) Screening and quantitative analysis for six sulfonylurea-type oral antidiabetic agents (SU-OADs), tolbutamide (TOL), acetohexamide (ACE), chlorpropamide (CHL), gliclazide (GLC), glibenclamide (GLB), and glimepiride (GLM), in adulterated health food has been developed. The SU-OADs were extracted with acetone and then the extract was subjected to thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In the TLC analysis, good separation was achieved with a mixture of n-butyl acetate containing 0.4% formic acid as a solvent. Ultraviolet irradiation at 254 nm was used to detect the SU-OADs. Specificity was obtained with Dragendorff’s test solution, 10% phosphomolybdic acid methanol solution, and 30% sulfuric acid methanol solu- tion. On the other hand, with a gradient reverse-phase HPLC system equipped with a photodiode array detector, we wer