丹参水溶性成分的纯化工艺和质量研究.pdfVIP

丹参水溶性成分的纯化工艺和质量研究 张秋燕、崔翰明、张春光、王阶* 中国中医科学院广安门医院 摘要 目的：提取纯化丹参中水溶性酚酸类有效成分，并进行相应的质量分析研究。方法： 以HPLC 检测丹酚酸 B 的含量，采用单因素和正交实验优化丹参的提取工艺；采用大孔吸 附树脂方法纯化丹参水溶性成分；并对丹参及其提取物的水溶性成分进行 HPLC 指纹图谱 研究，制定相应质量标准。结果：丹参中主要水溶性成分丹酚酸 B 的提取转移率达85% ， 提取纯化物中含丹酚酸B 大于65%、丹参素8%，其他丹酚酸12% 。相应HPLC 指纹图谱分 析方法可有效分析和鉴别主要水溶性特征峰。结论：本方法能够快速高效分离纯化丹参水溶 性成/组分，得到较高纯度的丹参总酚酸；HPLC 指纹图谱分析方法的精密度、重现性良好。 关键词：丹酚酸；柱层析；HPLC；指纹图谱 [Abstract] Objective: To extract and purify the active components of salvia miltiorrhiza, and to determinate the relevant quality analysis . Methods: The HPLC method of salvianolic acid Bwas established. The factors influencing the extraction were invested w ith a single-factor and orthogonal design. The water-soluble components of salvia miltiorrhiza were purified with macroporous resin . The HPLC fingerprint of Salvia miltiorrhiza and its purified extract were study, and the quality specification was established. Results: The transfer rate of salvianolic acid B was up to 85%, and the contents of salvianolic acid B and tanshinol were respectively 65% and 8% ， and other salvianolic acid about 12%. The HPLC fingerprint analysis method can effectively analyze and identify the main characteristic peaks of water-soluble components. Conclusion: This method can quickly and efficiently purified Salvia miltiorrhiza water-soluble components. The salvianolic acid components that had higher purity were obtained. The HPLC fingerprint method has good precision and reproducibility. Key Words: salvianolic acid ；column chromatography ；HPLC；fingerprint 丹参为唇形科鼠尾草属植物丹参（Salvia miltiorrhiza Bunge）的干燥根及根茎。丹 参性苦，微寒；归心、肝经；具有祛瘀止痛，活血通经，清心除烦等功效。是一味普遍用于 心血管疾病治疗的传统中药。现代研究发现丹参的有效成分主要分为两大类：丹酚酸类和丹 [1] 参酮类。丹酚酸类化合物以抗氧化、抗凝血和细胞保护作用为主 ；而丹参酮类化合物以改 [2]