Effects of Dietary β-Glucan and Glycyrrhizin on Non-Specific Immunity and Disease Resistance of the Sea Cucumber (Apostichopus japonicus Selenka)Challenged with Vibrio splendidus.pdfVIP
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Effects of Dietary β-Glucan and Glycyrrhizin on Non-Specific Immunity and Disease Resistance of the Sea Cucumber (Apostichopus japonicus Selenka)Challenged with Vibrio splendidus
J.OceanUniv.China (OceanicandCoastalSeaResearch)
D0I10.1007/s1l802—010一l757—2
ISSN 1672—5182,20109(4):389—394
http://www.oucedu.cn/xbywb/
E—mail.xbywb OblC.educn
ImmunityandDiseaseResistanceoftheSeaCucumber
f
usiaooonnticcuussSeelleennKkaa)ChnaanlleennRReead
CHANG Jie), ZHANGW enbing ,MAIKangsen¨MA Hongming¨andXU Wei)
, , ,
11TheKeyLaboratoO,ofMariculture,MinistryofEducationofChina,OceanUniversityofChina,Qingdao266003,尸R.China
21SchoolofAnimalScienceandTechnology,InnerMongoliaUniversityfortheNationalities,Tongliao028000, R.China
(ReceivedMarch31,201O:revisedMay18,2010;acceptedJuly1,2010)
@ OceanUniversityofChina.SciencePressandSpringer—VerlagBerlinHeidelberg2010
Abstract SeacucumbersApostichopus]aponicusSelenka,werefeddietscontainingnon—immunostimulant(basaldiet),0.2%
13-glucanand0.02%glycyrrhizininarecirculatorywatersystemfor45days,andsubsequentlychallengedwithVibriosplendidusby
inectionat1.0×10cfu/seacucumberforl5days.Phagocyticcapacity(PC),intracellularsuperoxideanionproduction(ISAP),ly—
sozyme(LSZ1activityandsuperoxidedismutasefSOD)activityinthecoelomicfluidwereanalyzedonthe0,5,l0“and15“days
afteriniection.Resultsshowedthatafterthe45一dayfeedingperiod,PC,ISA LSZactivityandSODactivityinseacucumbersfed
withdietary 3-glucanorglycyrrhizinweresignificantlyhigherthaninthosefedwiththebasa1diet.Onthe5 dayafterinfection.all
theimmuneparametersexaminedintheseacucumbersinjectedwith splendidusdecreasedinvaluesignificantly.Onthe15“day,
PC.ISAPandLSZactivityreturnedto1evelssimilartothoseonthe0“day.Fortheseacucumbersinjectedwithsaline,therewere
nosignificantdifferencesinalltheimmuneparametersexaminedandinthecumulativemorbidityduringthe15-daychallengingtria1
Afterinjectingwith splendidus.thecumulativemorbidityofseacucumbersfedwiththebasaldietwassignificantlyhigherthan
thosefedwithdietary 3-glucanorglycyrrhizinwhenchallengedw
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