浅析4种黄酮类中药有效成分BCS分类及吸收机制.docVIP

精品论文 参考文献 浅析4种黄酮类中药有效成分BCS分类及吸收机制 哈尔滨市呼兰区第二人民医院 150518 【摘 要】研究分析4种黄酮类中药有效成分BCS分类及吸收机制。该研究根据生物药剂学分类系统（biopharmaceutics classification system，BCS），研究山柰酚、橙皮苷、芹菜素、染料木素等4种黄酮类中药有效成分的溶解性和渗透性，并对其进行BCS分类；同时对其吸收机制进行研究。参照《中国药典》2010年版中溶解度测定方法对4种黄酮类成分进行溶解度的测定，采用体外细胞培养法建立Caco2细胞模型，利用MTT法筛选出合适的给药浓度进行细胞转运实验来检测表观渗透系数（Papp）以判断渗透性，并根据BCS对其进行分类；采用Caco2细胞模型，选择高、中、低3组不同浓度化合物进行双向转运实验研究吸收机制。实验表明山柰酚、橙皮苷、芹菜素、染料木素等具有低溶解性、高渗透性，属于BCSⅡ类，其中山柰酚为主动转运吸收机制，而橙皮苷、芹菜素、染料木素等为被动吸收。该研究针对中药黄酮类单体有效成分的特性，对其溶解性及渗透性的评价方法进行了摸索，为进一步完善中药生物药剂学分类体系提供了理论依据。 【关键词】黄酮类中药有效成分；生物药剂学分类系统（BCS）；吸收机制；溶解性；渗透性 [Abstract] the research and analysis of the BCS classification and absorption mechanism of the active components of 4 kinds of flavonoids. According to the biopharmaceutics classification system（biopharmaceutics classification system，BCS），solubility of kaempferol，hesperidin，celery，genistein and four flavonoids effective components of traditional Chinese medicine and permeability，and the BCS classification. At the same time，the absorption mechanism were studied. Solubility in Chinese Pharmacopoeia 2010 Edition reference method for the determination of the solubility of 4 kinds of flavonoids determination using cell culture method in vitro Caco-2 cell model was constructed，using MTT method selected suitable drug concentration of cell transfer experiments to detect the apparent permeability coefficient（Papp）to determine the permeability，and BCS carries on the classification according to；the Caco2 cell model，selection of high，middle and low groups with different concentrations of compounds for bi-directional transport experimental study on absorption mechanism. Experiment table Mingshan kaempferol，hesperidin，celery，genistein and having a low solubility，high permeability，belonging to the BCS class II，including kaempferol for active transport absorption mechanism，and hesperidin，celery，genistein and for passive absorption. The study for herbal flavonoid monomer effective components characteristic，on the solubility and permeab