海洋赤潮藻球形棕囊藻在氮磷富营养下的细胞增殖（英文）.pdfVIP

生态环境学报 2009, 18(2): 447-452 Ecology and Environmental Sciences E-mail: editor@ Cell proliferation of Phaeocystis globasa, a red tide causative marine microalga in various phosphorus(P)- and nitrogen(N)-replete conditions * Cai Zhuoping, Huang Weiwei, Duan Shunshan Institute of Hydrobiology, Jinan University, Guangzhou 510632, China Abstract: Phaeocystis globosa, a prevalent bloom-forming microalgal species was grown in various phosphorus(P)-replete and ni- -3 -1 -3 -1 -3 -1 -3 -1 trogen(N)-replete conditions, 1P1N(f/2 medium): P=5×10 g·L , N=75×10 g·L ; 3P1N: P=15×10 g·L , N=75×10 g·L ; 1P3N: -3 -1 -3 -1 -3 -1 -3 -1 P=5×10 g·L , N=225×10 g·L and 3P3N: P=15×10 g·L , N=225×10 g·L , and its cell growth was measured by using chloro- phyll fluorescence determination and cell-counting methods. The results showed that growth curves of Phaeocystis globosa exposed each nutrient conditions exhibited as “S-shaped” curves through out the experiment, indicating the microalgal cells experienced three growth stages, namely slow-growth stage, fast-grow stage and stationary-growth stage. Chlorophyll fluorescence was affected obvi- ously by the P and/or N concentrations. Significantly higher chlorophyll fluorescence was observed in the 3P1N, 1P3N and 3P3N (above 900 μg·L-1) as compared with than that in 1P1N(only 850 μg·L-1), but there were