柿子皮提取物的抗氧化活性-食品科学.PDFVIP

※基础研究 食品科学 2011, Vol. 32, No. 05 45 贾贵东，杨建雄 * ，王 芳，徐 乐，包 闽 ( 陕西师范大学生命科学学院，陕西 西安 710062) 为评价柿子皮提取物(persimmon peel extracts ，PPE) 的抗氧化活性，采用不同的抗氧化评价方法以及 Fe2+ -H2 O2 或 Fe2+ -VC 诱导的离体鼠肝线粒体损伤模型。结果显示：PPE 具有良好的总抗氧化活性、还原力、脂质 过氧化抑制力以及自由基清除能力；清除 DPPH 自由基、超氧阴离子自由基(O2 ·) 、羟自由基( ·OH) 的IC50 值分 别为 57 、137、425 μg/mL ；另外，PPE 能显著地抑制线粒体脂质过氧化、肿胀、蛋白氧化和ATPase 活性降低。 说明 PPE 有明显的体外抗氧化活性，有可能作为一种天然抗氧化剂的新原料。 柿子皮；抗氧化；自由基；线粒体 Antioxidant Activity of Persimmon Peel Extracts JIA Gui-dong ，YANG Jian-xiong* ，WANG Fang ，XU Le ，BAO Min (College of Life Science, Shaanxi Normal University, Xi ’an 710062, China) Abstract：In order to evaluate the antioxidant activity of persimmon peel extracts (PPE) in vitro, different antioxidant evaluation assays and a mouse model of liver mitochondrial damage induced by Fe2+-H2O2 or Fe2+-VC in vitro were used. The results showed that PPE possessed excellent total antioxidant activity, reducing power, inhibitory activity against lipid peroxidation and radical scavenging activity. The IC50 values against DPPH, superoxide anion and hydroxyl radicals were 57, 137 μg/mL and 425 μg/mL, respectively. In addition, PPE could significantly protect mitochondria from lipid peroxidation, swelling, protein oxidation and the reduction of ATPase activity in rats. In conclusion, the current study showed that PPE can resist oxidation obviously in vitro and be utilized as a new natural source of antioxidant. Key words ：persimmon peel ；antioxidant ；free radical ；mitochondria 中图分类号：R151.2 文献标识码：A 文章编号：1002-6630(2011)05-0045-05 [7]