1952年Berman2的试验开辟了人工食管研究的先河-第三军医大学学报.DOCVIP

京尼平交联脱细胞猪膀胱的体外生物学性能研究 范雪梅1，王颖楠1 ，陈卫军3 ，徐惠成1 （400038重庆，第三军医大学：西南医院妇产科1，基础医学部解剖学教研室，重庆市生物力学重点实验室2） ［摘要］目的 探讨新型生物交联剂京尼平对脱细胞猪膀胱性能的影响，为女性盆底功能重建提供理想的组织工程补片。方法 采用表面活性剂+酶消化法对猪膀胱进行脱细胞处理后，用0.625%京尼平溶液交联脱细胞膀胱72 h。通过HE、Weigert弹力纤维+VanGieson结缔组织（ET+VG）染色及扫描电镜进行组织形态观察，使用万能材料试验机检测材料生物力学性能，并对交联前后的脱细胞猪膀胱进行厚度、吸水性、体外胶原酶降解性能测定，以L929细胞为实验细胞，采用MTT法评估材料的细胞毒性。结果 京尼平交联脱细胞猪膀胱后呈深蓝色，保持了天然组织构架的完整，胶原纤维更加致密，吸水性提高，力学性能良好，脱细胞猪膀胱在第7天已完全降解，交联后在第28天降解率2%。交联前后材料的细胞毒性为0～1级。结论 经天然交联剂京尼平交联的脱细胞猪膀胱吸水性好，具有良好的生物力学性能，抗酶解能力强且无细胞毒性，是一种较为理想的盆底重建材料。 ［关键词］ 京尼平；脱细胞猪膀胱； 交联； 力学性能； 细胞毒性 ［中图分类号］:R318.08；R318.16；R711.2 【文献标志码】A Characteristics of Acellular Porcine Bladder Crosslinked with Genipin Fan Xuemei1,Wang Yingnan,Chen Weijun2,Xu Huicheng1（1Department of Obstetrics and Gynecology,Southwestern Hospital, 2Department of Anatomy, College of Basic Medical Sciences，Third Military Medical University,Chongqing,400038,China) ［Abstract］ Objective To investigate the characteristics and cytocompatibility of acellular porcine bladder fixed with genipin in vitro, and to provide ideal biomaterials for the reconstruction of female pelvis. Methods Cellular components of porcine bladder were removed by detergent-enzymatic method. The acellular porcine bladder was crosslinked with 0.625% genipin for 72h, then examined the morphology with hematoxylin and eosin staining, elastin/van Gieson staining and scanning electron microscope. Subsequently, the biomechanical tests were also performed. Properties of the crosslinked acellular matrix were evaluated by tissue thickness measurement, water absorption and in vitro enzymatic degradation test. The cytotoxicity of the genipin-fixed materials was evaluated in vitro by MTT assay using a murine fibroblast cell line L-929. Results The genipin-crosslinked acellular porcine bladder was dark blue, with very well preserved collagen fiber. Cosslinked tissues had strong hydrophilicity and good mechanical properties, the degradation ratio was only 2% after collagenase degradation for 28 days.