天龙咳喘灵对缺氧性肺动脉高压大鼠一氧化氮合酶及其基因表达的实验研究.docVIP

精品论文 参考文献 天龙咳喘灵对缺氧性肺动脉高压大鼠一氧化氮合酶及其基因表达的实验研究 王 文 　　广州医学院第一附属医院 广东广州 510000 　　【摘 要】目的：探讨天龙咳喘灵对缺氧性肺动脉高压大鼠一氧化氮合酶（NOS）及其基因表达的影响。方法：以45只SD大鼠为研究对象，随机均分为三组：A组正常饲养，做健康对照；B组置入常压低氧舱，10h/d，持续4周，做疾病对照；C组同种方案置入常压低氧舱，并予天龙咳喘灵灌胃。4周后测定大鼠肺动脉压（mPAP）、动脉血NO水平、肺动脉cNOS及iNOS活性、肺动脉cNOSmRNA及iNOSmRNA表达。结果：mPAP对比，B组明显高于A、C组（P＜0.05），C组略高于A组，但差异不显著（P＞0.05）；动脉血NO水平对比，B组均明显低于A、C组（P＜0.05），C组略低于A组，但差异不显著（P＞0.05）；iNOS及其mRNA对比，均呈现C组高于B组高于A组趋势，两组间差异均有统计学意义（P＜0.05）；cNOS及其mRNA对比，均呈现C组高于A组高于B组趋势，两组间差异均有统计学意义（P＜0.05）。结论：天龙咳喘灵有助于降低缺氧性肺动脉高压大鼠平均肺动脉压、提升其动脉血NO水平，这可能与其能够显著提升大鼠NOS及其基因的表达水平有关。 　　【关键词】天龙咳喘灵；缺氧性肺动脉高压；一氧化氮；一氧化氮合酶；大鼠 　　【中图分类号】R972+.6 【文献标识码】B 【文章编号】1764-8999（2015）7-0573-02 　　【Abstract】Objective：To investigate the Denon Kechuan Ling on hypoxic pulmonary hypertension rats, nitric oxide synthase (NOS) gene expression and its influence. Methods 45 SD rats as the research object, randomly divided into three groups: A group with normal feed, do healthy controls; B group was implanted with low oxygen chamber at atmospheric pressure, 10h/d, for 4 weeks, as disease control; group C the same scheme of atmospheric low oxygen chamber implantation, and to Denon Kechuanling gastric lavage. Rat pulmonary artery pressure were measured after 4 weeks (mPAP), the expression level of arterial blood NO, cNOS and iNOS activity in pulmonary artery, pulmonary artery cNOSmRNA and iNOSmRNA. Results：mPAP comparison, B group was significantly higher than that in A, C group (P lt; 0.05), C group was slightly higher than that of A group, but the difference was not significant (P gt; 0.05); the contrast level of arterial blood NO, A, B were significantly lower than those in C group (P lt; 0.05), C group was slightly lower than that of group A, but the difference was not significant (P gt; 0.05); iNOS and mRNA contrast, showed a C group than in B group than in A group trends, differences between the two groups was statistically significant (P lt; 0.05); cNOS and mRNA contrast, showed a C group than in A group than in B gro