微泡造影剂联合高强度聚焦超声阻断毫米级兔股动脉血流的实验研究.docVIP

微泡造影剂联合高强度聚焦超声阻断毫米级兔股动脉血流的实验研究岳渝娟1 ,陈文直2,王智彪2（重庆医科大学附属第一医院肿瘤科；2超声工程研究所，重庆 400016） 摘 要： 目的 探讨微泡造影剂联合高强度聚焦超声（igh intensity focused ultrasound, HIFU）通过体外照射能否毫米级兔股动脉内血栓形成，血流被较长期阻断。方法 将96只兔随机分成单纯照射组和微泡造影剂联合照射组照射后即日、3 d、7 d用HE染色观察各组兔股动脉组织学变化，DSA观察兔股动脉血流变化。结果微泡造影剂联合照射组各观察时间点所有兔股动脉内均可见血栓形成，血流中断；单纯照射组兔股动脉内未见血栓形成及血流中断。结论 微泡造影剂能增强空化效应，使毫米级的兔股动脉在较长的时间内（7 d）血栓形成，血流中断。 关键词高强度聚焦超声；微泡造影剂；血栓；兔；股动脉Experimental study on occluding blood flow in femoral artery of rabbit after high intensity focused ultrasound combined with microbubble agent exposure Yue Yu-juan1，（Department of Oncology,2 Institute of Ultrasonic Engineering，The First Affiliated Hospital of Chongqing Medical Univeristy, Chongqing 400016, China） AbstractObjectives To investigate whether high-intensity focused ultrasound ( HIFU) combined with Microbubble Agent can make the femoral artery of rabbit forming thrombus inside and occlude the blood flow for a little long time after exposure. Methods Ninety-six rabbits were divided into two groups randomly .A group received only HIFU exposure. The other group were irradiated with HIFU combined with Microbubble Agent. After exposure，HE staining were used to observe the histopathologic change of the femoral artery, digital subtraction radiography（DSA） was used to observe the change of the blood flow. Results There were thrombus formed in the femoral artery and the blood flow was occluded in every rabbit irradiated with HIFU combined with Microbubble Agent. Conclusions 作者简介：岳渝娟，女，重庆市人，硕士研究生，医师，主要从事肿瘤综合治疗方面的研究E-mail：grass220@163.com Thrombus could be formed in the femoral artery and blood flow occlusion had been accomplished by HIFU combined with Microbubble Agent for at least 7 days. Key words: High-intensity focused ultrasound；Microbubble Agent； Blood vessel embolism；Rabbi；Femoral artery 阻断动脉血流是一种重要的临床治疗手段，可以治疗或辅助治疗多种疾病， HIFU可以暂时（﹤24 h）阻断亚毫米级和毫米级动脉血流 [13]，本实验探讨微泡造影剂联合HIFU照射能否使毫米级的兔股动脉[3]血流被较长期阻断。 1.材料方法 1.1 实验动物与分组 纯种健康新西兰兔96只，3月龄，体质量1.5～2.0 kg，雌雄不限。由重庆医科大学实验动物中心提供。将96只兔随