陈纯-四种浮游植物生物量计算方法的比较分析_定稿陈纯-四种浮游植物生物量计算方法的比较分析_定稿.pdfVIP

四种浮游植物生物量计算方法的比较分析 1 1 1,2 1,2 陈纯 ，李思嘉 ，胡韧 ，韩博平 （1：暨南大学生态学系，广州 510632） （2：水体富营养化与赤潮防治广东普通高校重点实验室，广州 510632） 摘 要：浮游植物是水生生态系统中重要的初级生产者，其生物量是反映其现存量的关键指标。本文利用具有 3 个处理组 的大型围隔实验中浮游植物数据，对文献中常见的计算浮游植物的种群生物量和群落生物量的四种方法：标准法、细分 法、粗分法和资料法进行比较，并分析采用这 4 种不同方法得到的浮游植物生物量与叶绿素 a 浓度的相关性。结果表明： 粗分法是计算浮游植物生物量的高效方法，能够保证准确度和节省时间；提高浮游植物生物量计算确准度不是影响浮游 植物生物量与叶绿素 a 浓度相关性显著程度的关键。通过比较剔除稀有种（生物量不超过群落生物量的 5%的种类）前后 浮游植物生物量的差异，忽略稀有种会导致种类均匀度较高的浮游植物群落生物量严重偏低，建议浮游植物生物量的计 算不能一概忽略稀有种。 关键词：浮游植物；生物量；计算方法；叶绿素a；稀有种 Comparative analysis of four methods in calculating phytoplankton biomass 1 1 1,2 1,2 CHEN Chun , LI Sijia , HU Ren HAN Bo-Ping (1: Department of Ecology, Jinan University, Guangzhou 510632, P.R. China) (2: Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Guangzhou 510632) Abstract: Phytoplankton is the important primary producer ，its biomass measures its standing stock in aquatic ecosystem. However, the error in calculating the biomass is unclear in different methods. Based a data set of phytoplankton from an enclosure experiment with three treatments covering a series of phytoplankton communities, we compared phytoplankton community biomass and phytoplankton species population biomass by four calculating methods which are widely applied in China, i.e., Standard method, Fine-group method, Coarse-group method and Literature method, and investigated the correlation between chlorophyll a concentration and the phytoplankton biomass calculated by the four methods. The results showed that the coarse-group method is a high-efficiency way to calculate phytoplankton biomass and suggested to apply in calculating phytoplankton biomass. The positive correlation between phytoplankton biomass and