武汉百意欣cAMP包被羊抗鼠IgG直接竞争ELISA检测说明书.pdfVIP

Direct Competitive cAMP ELISA Kit Based on Monoclonal Anti-cAMP Antibody Introduction Adenosine 3, 5-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC (50% B/B ) of approximately 12 pmol/mL and a detection 50 0 limit of approximately 0.15 pmol/mL. Kit Components 1. cAMP standard Cat.No: Bk0 11 10000 pmol/mL cAMP, 1x0.1mL 2. cAMP-HRP Conjugate Cat.No: Bk0 12 1000x stock of HRP conjugated with cAMP, 1x0.02mL 3. cAMP Antibody Cat.No: Bk0 13 Anti-cAMP monoclonal antibody (1000X stock) , 1x0.02mL 4. Assay Buffer(10x) Cat.No: Bk004 phosphate buffer with preservative, 1x30mL 5. Neutralizing Reagent Cat.No: Bk005