HPLC测定扶正柔肝颗粒柯里拉京 和丹酚酸B的含量.pdfVIP

Pharmacy Information 药物资讯, 2015, 4(4), 43-49 Published Online November 2015 in Hans. /journal/pi /10.12677/pi.2015.44006 Determination of Corilagin and Salvianolic Acid B in Fu Zheng Rou Gan by HPLC 1 1* 1 2 2 Lifei Song , Changqing Liu , Mansha Li , Jianjun Yang , Changqing Li 1 Guangzhou Zeli Pharmatech Co., Ltd., Guangzhou Guangdong 2 Guangzhou Zhongze Biological Technology Co., Ltd., Guangzhou Guangdong nd rd th Received: Nov. 2 , 2015; accepted: Nov. 23 , 2015; published: Nov. 27 , 2015 Copyright © 2015 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract Objective: To establish a high liquid chromatography method of determinating Corilagin and Sal- vianolic acid B in Fu Zheng Rou Gan. Method: Chromatographic column: InertsiL® ODS-SP (5 mi- crons, 4.6 × 250 mm). The mobile phaseis is acetonitrile-0.2% phosphoric acid (gradient elution) with detection wavelength 210 nm; the flow rate is 1.0 mL/min; the column temperature is 35˚C and the inject volume is 5 μL. Result: In the range of 0.061 - 0.510 μg, the linear relationship of Co- rilagin is good (r = 0.9997), and the average sample recovery rate is 101.41% (RSD = 1.68%, n = 6). In the range of 0.504 - 4.203μg, the linear relationship of Salvianolic acid B is also good (r = 0.9999), and the average sample recovery rate is 103.30% (RSD = 1.11%, n = 6). Conclusion: The method is sensitive, rapid and accurate and can be used for quality control of this granule. Keywords Fu Zheng Rou Gan, Content Determination, Corilagin, S