一种圆形横截面PDMS体外微血管模型构建.DOC

一种圆形横截面PDMS微血管模型的构建( 李远1 , 刘北忠1, 龚放1, 于超2, 廖娟1 ,胡礼仪1 * 1 重庆医科大学附属永川医院中心实验室，402160，重庆 2 重庆医科大学生命科学研究院，400016，重庆 摘要：基于软刻工艺发展的微血管模型为体外研究微循环系统疾病病理机制提供了新思路，然而标准的软刻工艺加工的微通道呈矩形截面，不能真实地反应体内微血管管腔的圆形截面特征。本文介绍了一种简易的构建具有圆形截面特征聚二甲基硅氧烷内皮细胞EA.hy926, Chongqing Medical University, Chongqing, 400016, China Abstract: Microvascular model based on soft-lithography technology has provided a new strategy for researching the pathological mechanism of microcirculatory system in vitro; however, the rectangular microchannel fabricated by standard soft-lithography technology not truly mimic the feature of circular cross section of microvascular tube in vivo. This article presents a simple method for building a Polydimethylsiloxane(PDMS) microvascular model with circular cross section. This method used micro stainless steel wire with circular cross section as the mold, on which PDMS was poured, then the straight microchannel with circular cross section was formed after the micro wire was draw out from the cured PDMS. The formed microchannel was used as a perfusion chamber for culturing the EA.hy926 vascular endothelial cells EA.hy926 in the microchannel reached confluence within 96h and could withstand a 25dyn/cm2 fluid shear stress. This method, which can be used to develop a circular cross section PDMS microvascular model, is rapid and simple. In future, the developed microdevice can be used for microcirculatory system studies in vitro. Key words: Cell culture technique; Microfluidic chip; Microchannel; PDMS; Microvascular model Supported by the Natural Science Foundation of Chongqing(cstc2012jjA10046) , Health Bureau Medical research project of Chongqing(2012-2-165) and Yongchuan Science and technology plan projects (YCSTC,2012BE5025) Corresponding author: Liu Beizhong, Tel:023E-mail: lbzemail@163.com 1. 引言 基于软刻工艺发展出的微流控芯片技术为体外构建不同空间拓扑结构和表面性质的微血管模型提供了新策略，并已成为体外研究微循环系统疾病相关病理生理机制的强大工具[1-3]。利用微流控芯片技术进行微循环系统疾病的研究具有可实现流体入口边界条件的精确控制，采用高分辨率显微镜成像技术能实时观察、量化微通道网络内的细胞和粒子的流动行为等优点[4]。基于软刻工艺的