不同化感潜力水稻根际土壤微生物生理特性分析-应用与环境生物学报.docVIP

田间直播下化感水稻抑草作用与根际土壤微生物生理特性动态分析* 张 奇,2 王海斌,3 李 立 陈 尧 李家玉,2 方长旬,2 何海斌,2** 1福建省农业生态过程与安全监控重点实验室，福建农林大学生命科学学院 福州 350002 作物生态与分子生理学福建省高校重点实验室，福建农林大学 福州 350002 364012 摘要 考察水稻根际土壤微生物特性变化，本研究在人工除草与不除草的田间种植模式下，化感水稻PI312777和非化感水稻Lemont苗期对田间杂草的抑制作用根际土壤的化感潜力以根际土壤微生物生理特性动态。结果表明：PI312777的田间抑草率在7叶期达85.82%土壤－琼脂三明治法实验表明PI312777根际土壤稗草抑草率显著高于20.16%。在相同叶期下PI312777的根际土壤微生物碳量及呼吸强度细菌数量和土壤酶脲酶、蛋白酶和蔗糖酶活性均远高于Lemont，且不除草处理下显著高于除草处理。PI312777根际土壤抑草率表明了根际微生物活动关系紧密化感物质土壤微生物变化化感作用是一个植物－土壤之间的相互作用过程7) 关键词 化感水稻化感作用根际土壤微生物生理特性土壤酶活性 ynamic analysis on weeds inhibition of allelopathic rice and microbial physiological traits of rice rhizospheric soils in field test* ZHANG Qi1,2, WANG Haibin1,3, LI Li1, CHEN Yao1, LI Jiayu1,2, FANG Changxun1,2, HE Haibin1,2** 1Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China 2Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 35002, China 3 College of Life Sciences, Longyan University, Longyan 364012, China Abstract In order to explore the dynamic change of microbial physiological traits in rhizospheric soils of allelopathic rice cultivars, weeds inhibition of allelopathic rice PI312777 and nonallelopathic rice Lemont, allelopathic potential of rice rhizospheric soils, as well as the microbial physiological traits of rice rhizospheric soils, were studied in field tests under weed-removing treatment and without weed-removing treatment. The results showed that the inhibitory rate of PI312777 at 7-leaf stage on paddy weeds was 85.82%. By Soil-Agar Sandwich method, allelopathic potential of PI312777 rhizospheric soils on barnyard grass was significant higher at 5-leaf stage than that at 3-leaf stage, and was increased by 20.16% from 3-leaf stage to 5-leaf stage under without weed-removing treatment. When at the same leaf stage, the soil microbe biomass car