免疫金银染色法快速检测水产品中孔雀石绿残留的研究.PDFVIP

8 6 Vol. 8 No. 6 2017 6 Journal of Food Safety and Quality Jun. , 2017 * 付林峰, 汪 丽, 娄 越, 简锦明, 郭希山 , 朱松明 (, 310058) 摘 要: 目的 方法 , , (nitrocellulose, NC), , , 结果 20 nm , pH 9.0, 1 mL 15 μg 1:10 20 μL PBS (0.01 mol/L, 10% , 0.05% -20) , NC 3 mg/mL 0.7 mg/mL , 0.2 ng/mL, 10 min结论 , 90 d , 关键词: ; ; ; ; Rapid detection of malachite green residues in aquatic products with immunogold-silver staining method * FU Lin-Feng, WANG Li, LOU Yue, JIAN Jin-Ming, GUO Xi-Shan , ZHU Song-Ming (College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China) ABSTRACT: Objective To develop a rapid, steady detection method of malachite green residues in the aq- uatic product. Methods A colloidal gold immunochromatographic assay strip was developed for rapid detec- tion of malachite green (MG) residues in aquatic products and immunogold-silver staining method was em- ployed to improve the sensitivity of the assay strip. This method was based on colloidal gold immunochroma- tography technology which involved the competition method and used nitrocellulose (NC) membrane