米心水青冈遗传多样性分析报告.docVIP

米心水青冈遗传多样性和遗传结构研究 摘要： 利用6对SSR引物对米心水青冈的25个居群的遗传多样性及遗传结构进行了检测分析，结果表明，米心水青冈的遗传多样性（Na= 47，He=0.576）偏低，供试居群的基因分化系数Fst 平均为0.1385，表明14%的遗传变异存在于居群间，而86%的遗传变异存在于居群内Genetic diversity and genetic structure of Fagus engleriana Seem.( Fagaceae) Abstract: Genetic diversity and genetic structure of 25 populations of Fagus engleriana Seem. were studied by using 6 microsatellite loci. he results revealed a low degree of genetic diversity (Number of different alleles, Na was 4. 7. He was0.576).The genetic differentiation (Fst=0.1385)suggested that 14% genetic variation existed among populations ,meaning that 86%genetic variationhappened among the individuals within populations.the cluster analysis showed that genetic identity(0.0656 to0.7420 ) among populations was negatively correlated with the geographical distance ,consistent with the isdation by distance model for the isolation populations. The results are useful for providing scientific basis for the assessment for genetic diversity and appropriate conservation strategies of Fagus engleriana. Key words ：Fagus engleriana；Genetic diversity；Genetic structure；SSR 近年来，SSR作为分子标记所具有的优点使其在植物遗传图谱构建及基因或QTL的定位、物种进化与分类、品种遗传多样性、品种保护与亲子鉴定、纯度鉴定、杂种优势的机理及其预测等方面得到广泛应用[1]。与RAPD技术及AFLP技术相比，微卫星技术在分析遗传多样性方面更具有优势，现已被广泛应用于农作物[2]、畜牧[3]等的分析中。分析过去的历史和现在的过程（如漂变、扩散、交配和选择、居群大小，基因流），分子标记居群遗传结构是最具价值的[]。水青冈属植物是北半球重要建群种[]，根据米心水青冈的分布特点，在安徽、浙江、湖北、湖南、四川、重庆、陕西、甘肃、广西、云南10个省市（自治区）共获得米心水青冈25个居群526份样本[6]法提取DNA，并用1%琼脂糖凝胶电泳检测，在紫外分析仪上观察拍照。DNA主带清晰、无弥散带、RNA去除干净则认为DNA质量较高。 1.3 SSR引物 从四篇文献中筛选引物：日本波叶水青冈（F. crenata）9对引物[]，欧洲水青冈（F. sylvatica）6对引物[]，日本波叶水青冈（F. crenata）16对引物[]，长柄水青冈（F. longipetiolata）11对引物[]。总共52对引物进行筛选Locus Primer sequences（5’-3’） Repeat motif Ta（℃） Size range（bp） sfc0036 F: CATGCTTGACTGACTGTAAGTTC (TC)23 60 96-142 R: TCCAGGCCTAAAAACATTTATAG sfc0305 CCAATGGACTTGTTATACCAATC (GA) 24 60 159-203 GCACCAGTTGCTTACAGAATAG sfc1143 TGGCATCCTACTGTAATTTGAC (AG) 21 60 96-136 ATTCCACCCACCATCTGTC Fl05 GGCACATTACACCTAAATA (TC) 12 59 153-189 GCATAATAAAGAGAGG