红外光谱结合系统聚类分析对云南产石斛的研究-中国中医药信息杂志.pdfVIP

2014年 9月第 21卷第 9期 中国中医药信息杂志 ·75· 红外光谱结合系统聚类分析对云南产石斛的研究 1 2 杨永荣 ,刘飞 1.玉溪市人民医院医务部,云南 玉溪 653100；2.玉溪师范学院物理系,云南 玉溪 653100 摘要：目的 采用傅里叶变换红外光谱技术结合系统聚类分析对云南产石斛进行研究。方法 用傅里叶变 换红外光谱法测试了 11个品种83株石斛样品的红外光谱,并结合二阶导数光谱对比分析光谱信息差异,用相关 分析和系统聚类分析进行品种差异和鉴别分析。结果 光谱信息显示,石斛中的主要成分是纤维素和木质素等 多糖物质；铁皮石斛中纤维素和木质素的含量相对较小,其他多糖物质成分及含量相对较多。测试石斛品种的 相关性较高,红外光谱的相关系数均在 0.980 以上,二阶导数光谱的相关系数在 0.751～0.980 之间。用二阶导 数光谱进行系统聚类,分类正确率达 87.9%；系统聚类显示重唇石斛、罗河石斛与钩状石斛,长苏石斛与金钗石 斛,滇桂石斛与流苏石斛的物质组分更相近。结论 红外光谱结合系统聚类分析可以区分不同品种石斛的物质 组分差异,鉴别不同品种的石斛,具有方便快捷的特点。 关键词：红外光谱；系统聚类；石斛 DOI：10.3969/j.issn.1005-5304.2014.09.022 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2014)09-0075-05 Study of Dendrobium from Yunnan by Fourier Transform Infrared Spectroscopy Combined 1 2 with Hierarchical Cluster Analysis YANG Yong-rong , LIU Fei (1.Medical Administration Division, Peoples Hospital of Yuxi City, Yuxi 653100, China ；2.Department of Physics, Yuxi Normal University, Yuxi 653100, China) Abstract ：Objective To study Dendrobium from Yunnan Province by Fourier transform infrared spectroscopy (FTIR) combined with hierarchical cluster analysis. Methods Stems of 83 trees from 11 Dendrobium varieties were tested through using FTIR, so as to analyze the differences among different varieties and identify Dendrobium variety by second-derivative spectral, the correlation analysis and hierarchical cluster analysis. Results The spectra showed that the main ingredients of stems were cellulose, lignin and polysaccharides. The fraction of lignin and cellulose of D.officinate was lower, while that for polysaccharides was higher. There was a high correlation among spectra, with the correlation coefficients of more than 0.980, and th