超微量蛋白分析仪在微量树突细胞中ERK蛋白及其磷酸化修饰检测中的应用研究.pdfVIP

中国医学装备2017年2月第14卷第2期 China Medical Equipment 2017 February Vol.14 No.2 学术论著 超微量蛋白分析仪在微量树突细胞中 ERK蛋白及其磷酸化修饰检测中的应用研究* 任怡然① 程雪莲① 梁昊岳① 白杨① 杨晚竹① 于文颖① 陈婷① 付伟超① 郭 丹①* [文章编号] 1672-8270(2017)02-0131-04 [中图分类号] R197.39 [文献标识码] A [摘要] 目的： 建立利用超微量蛋白分析仪检测微量细胞样品中低丰度细胞外调节蛋白激酶(ERK) 及其磷酸化修饰的应用方法，为在科研工作中应用该仪器提供实验依据。方法：采用配制样品溶 液，上样、分离、固定、免疫杂交和软件定量等实验步骤，进行化学发光检测，对不同数目树突 状细胞中ERK蛋白及其磷酸化修饰的定性及定量结果进行分析。结果：超微量蛋白分析仪能够 检测到低至2000个细胞中的目的蛋白并获得定量数据，该仪器还能够有效分辨出20000个细胞中 ERK蛋白不同形式的磷酸化修饰。结论：超微量蛋白分析仪有效完成了对低丰度细胞外调节蛋 白以及蛋白质翻译后修饰检测。 [关键词] 超微量蛋白分析仪；微量细胞；翻译后修饰；定量；化学发光 DOI: 10.3969/J.ISSN.1672-8270.2017.02.039 Application study of Nanopro 1000 in the detection of ERK protein in trace dendritic 作者简介 cells and its phosphorylation product/REN Yi-ran, CHENG Xue-lian, LIANG Hao-yue, et al//China Medical Equipment,2017,14(2):131-134. 任怡然,女,(1990- ), [Abstract] Objective: To understand the principles of Ultramicro-protein analyzer system 硕士研究生。技师。中 and provide a reference for using it by analyzing low abundance ERK in trace dendritic cell 国医学科学院血液病医 and its phosphorylation product. And to provide application experience of this equipment in 院血液学研究所 实验 science and research work. Methods: The qualitative and quantitative results of ERK and its phosphorylation product in different numbers of dendritic cells were analyzed by means of 血液学国家重点实验 preparation of sample, such as solution, instrument operation and software analysis. Result: 室，研究方向：基因与 Ultramicro protein analyzer system can detect target protein in as low as 2000 cells and obtain 蛋白相关仪器的应用。 quantitative data. The instrument also can efficiently distinguish different phosphorylation modification form of ERK in 20000 cells. Conclusion: This system is very helpful for the study of low abundance extracellular regulation protein and post-translational modification of proteins. [Key words] Nanopro 1000 sys