丰富环境对缺血缺氧脑损伤新生大鼠海马脑源性-中国康复医学杂志.pdfVIP

ChineseJournalofRehabilitationMedicine, Jul. 2017,Vol. 32, No.7 ·基础研究· 丰富环境对缺血缺氧脑损伤新生大鼠海马脑源性 神经营养因子表达及细胞凋亡的影响* 宋名杨 朱路文 叶 涛 姜云飞 赵 彬1 1 2 1 1，3 摘要 目的：探讨丰富环境对缺血缺氧脑损伤（HIBD ）新生大鼠海马脑源性神经营养因子（BDNF ）表达及细胞凋亡的影响。 方法：采用随机数字表法将36 只7 日龄新生大鼠分为假手术组、模型组及丰富环境组，各组又分为术后7d、28d 两个 亚组（n=6）。采用Rice-Vannucci 经典方法建立新生大鼠HIBD 模型。模型组及假手术组不予任何干预，丰富环境组 予以早期抚触及丰富环境刺激治疗。术后7d、28d 采用水迷宫检测方法评估大鼠学习记忆能力，免疫组化法检测大 鼠海马CA1 区BDNF 的表达，TUNEL 法检测海马CA1 区细胞凋亡。 结果：术后7d、28d ，与模型组比较，丰富环境组逃避潜伏期均缩短（P ＜0.05 ），穿越平台次数增多（P ＜0.05 ），BDNF 表达升高（P ＜0.05 ），TUNEL 阳性细胞数降低（P ＜0.05 ）。 结论：丰富环境能改善HIBD 新生大鼠学习记忆能力，其机制可能与上调海马区BDNF 的表达及抑制细胞凋亡有关。 关键词 丰富环境；缺血缺氧性脑损伤；脑源性神经营养因子；凋亡 中图分类号：R742.3 ，R722 ，R493 文献标识码：A 文章编号：1001-1242（2017 ）-07-0750-06 Effects of enriched environment intervention on brain-derived neurotrophic factor expression and apopto⁃ sis in hippocampus of hypoxic ischemic brain damaged neonatal rats/SONG Mingyang, ZHU Luwen, YE Tao,etal.//ChineseJournal ofRehabilitation Medicine, 2017,32(7): 750—755 Abstract Objective: To investigate the effect of enriched environment intervention on brain- derived neurotrophic factor expression and apoptosis in hippocampus of hypoxic-ischemic brain damaged(HIBO) neonatal rats. Method: Thirty-six male 7-day-old rats were randomly divided into sham operation group, model group, en- riched environment group, each group was further divided into 7 days and 28 days subgroups (n=6). HIBD model of neonatal rats was established according to Rice-Vannucci classical methods. The model group and the sham operation group accepted no treatment. Enriched enriched environment group was treated with early touch and enriched environment stimulation. 7 days and 28 days after operation, the learning and memory ability was evaluated with the water maze detection method, the expression levels of BDNF were detected by i