隐藏嗜酸菌acidiphiliumcryptumjf-5双组分信号转导系统余水静.docVIP

隐藏嗜酸菌Acidiphilium cryptum JF-5双组分信号转导系统 余水静 （江西理工大学资源与环境工程学院生物工程系，江西，赣州，341000） 摘要：隐藏嗜酸菌Acidiphilium cryptum）对多变极端环境条件感知和反应孤儿和个孤儿反应调节蛋白隐藏嗜酸菌 中图分类号：TF18 文献标识码：A wo-component signal transduction systems of Acidiphilium cryptum JF-5 Shuijing Yu, Yanping Peng, Yangwu Deng, Yanhua Guo and Heying Liu (Department of Biotechnology, School of Resources and Environment, Jiangxi University of Science and Technology, Ganzhou 341000, P. R. China) Abstract: In order to explore the molecular mechanism of Acidiphilium cryptum response to extreme conditions in the mine environment, we analyzed the distributions, structures and putative biological functions of two-component signal transduction systems (TCS) in A. cryptum JF-5. And 9 TCSs, 2 hybrid histidine kinases (HKs), 3 orphan HKs and 5 orphan response regulator proteins (RRs) were identified. Five TCSs were invovled into the transcription regulation of A. cryptum response to heavy metal. The N-terminal regions of most HKs were characterized by the presence of transmembrane helices, HAMPs, or PAS domains. More than 40 percent of putative RRs were classified into OmpR subfamily. The common clustering TCS genes in the same branch of the evolutionary tree may have the same evolutionary pathway. Our results should open a novel research direction in A. cryptum for molecular mechanism of the adaptation in the extreme environment. Keywords: Acidiphilium cryptum, Two-component signal transduction system, Histidine protein kinase, Response regulator protein, Domain architecture, Bioinformatics analysis. 作者简介：余水静，（1976-），男，，博士。研究方向：微生物冶金及基因组学 基金资助：江西省科技支撑计划项目资助（项目编号：20121BBG70004） 通讯地址：江西省赣州市江西理工大学资源与环境工程学院生物工程，邮编：341000电话：0797831231（办公室）；（手机） E_mail：yushuijing2008@ 隐藏嗜酸菌(Acidiphilium cryptum)是一种能利用有机物、H2、单质硫或者还原Fe3+而获得能量进行化能异养生长的嗜酸兼性异养细菌，为革兰氏阴性菌[1]，一般生长在酸性矿山废水、高温废水或一些重金属污染酸性矿环境中。隐藏嗜酸菌为金属还原细菌，能进行铁呼吸作用，使Fe3+还原成Fe2+，可使Cr6+还原成Cr3+降低Cr的毒性[2-3]。隐藏嗜酸菌还原Fe3+并利用其它浸矿细菌合成的有机物，通过这种方式，消除这些有机物的抑制和毒害作用，可为浸矿细菌如嗜酸氧化亚铁硫杆菌(Acidithiobacillus f