延胡索中9种生物碱对醌还原酶诱导活性研究-中国现代应用药学.pdfVIP

延胡索中9 种生物碱对醌还原酶诱导活性研究 施菁，李元圆，崔勤敏，陈婷，上官盈盈*(浙江医学高等专科学校药学系，杭州 310053) 摘要：目的 对延胡索的生物碱类成分及其醌还原酶(QR)诱导活性进行系统研究。方法 运用色谱学和波谱学方法分离 鉴定了9 个化合物的结构，并对分离得到的化合物单体进行QR 诱导活性测定。结果 从该植物中分得9 个化合物，分 别鉴定为d- 紫堇碱( Ⅰ)、去氢海罂粟碱( Ⅱ)、四氢巴马汀(Ⅲ)、原阿片碱(Ⅳ)、α-别隐品碱( Ⅴ)、四氢非洲防己碱(Ⅵ)、去 氢紫堇碱(Ⅶ)、小檗碱(Ⅷ)、巴马汀(Ⅸ)。QR 诱导活性筛选结果表明d-紫堇碱( Ⅰ)和四氢非洲防己碱(Ⅵ)显示了一定的QR 诱导活性。结论 首次对延胡索中的生物碱单体进行了QR 诱导活性测定，考察结果具有实际指导意义。 关键词：延胡索；生物碱；醌还原酶 基金项目：浙江省科技厅分析测试基金(2008F70017) 作者简介：施菁，女，硕士，副教授 Tel: (0571E-mail: shij136@ *通信作者：上官盈盈，女，教授 Tel: (0571E-mail: ying-ying6@163.com ·290 · Chin JMAP, 2011 April, Vol.28 No.4 中国现代应用药学2011 年4 月第28 卷第4 期 中图分类号：R285.5 ；R284.2 文献标志码：A 文章编号：1007-7693(2011)04-0290-05 Study on Nine Alkaloids from Corydalis Rhizoma with Quinone Reductase Inducing Activities * SHI Jing, LI Yuanyuan, CUI Qinmin, CHEN Ting, SHANGGUAN Yingying (Department of Pharmacy, Zhejiang Medical College, Hangzhou 310053, China) ABSTRACT: OBJECTIVE To study the chemical constituents from Corydalis Rhizoma and their quinone reductase(QR) inducing activties in vitro. METHODS Chromatographic and spectroscopic methods were used to isolate and identify the alkaloids from the ethanol extraction of Corydalis Rhizoma. QR induction assay was employed to investigate the QR inducing activities of the isolated alkaloids. RESULTS Nine compounds were isolated and identified as d-corydaline( Ⅰ), dehydroglaucine( Ⅱ), tetrahydropalmatin(Ⅲ), protopine(Ⅳ), α-allocryptopine( Ⅴ), tetrahydrocolumbamine(Ⅵ), dehydrocorydaline(Ⅶ), berberine(Ⅷ), palmatine(Ⅸ). Among these alkaloids, d-corydaline( Ⅰ) and tetrahydrocolumbamine(Ⅵ) possessed moderate QR inducing activities in vitro. CONCLUSION The QR inducing activities of alkaloids from Corydalis Rhizoma were investigated for the first time, and the results generally show a positive significance